Ramanathan Gajalakshmi, Craver-Hoover Brianna, Arechavala Rebecca J, Herman David A, Chen Jane H, Lai Hew Yeng, Renusch Samantha R, Kleinman Michael T, Fleischman Angela G
Division of Hematology/Oncology, Department of Medicine, University of California, Irvine, CA 92697, USA.
Department of Biological Chemistry, University of California, Irvine, CA 92617, USA.
Cancers (Basel). 2020 Aug 14;12(8):2292. doi: 10.3390/cancers12082292.
Electronic cigarettes (E-cigs) generate nicotine containing aerosols for inhalation and have emerged as a popular tobacco product among adolescents and young adults, yet little is known about their health effects due to their relatively recent introduction. Few studies have assessed the long-term effects of inhaling E-cigarette smoke or vapor. Here, we show that two months of E-cigarette exposure causes suppression of bone marrow hematopoietic stem and progenitor cells (HSPCs). Specifically, the common myeloid progenitors and granulocyte-macrophage progenitors were decreased in E-cig exposed animals compared to air exposed mice. Competitive reconstitution in bone marrow transplants was not affected by two months of E-cig exposure. When air and E-cig exposed mice were challenged with an inflammatory stimulus using lipopolysaccharide (LPS), competitive fitness between the two groups was not significantly different. However, mice transplanted with bone marrow from E-cigarette plus LPS exposed mice had elevated monocytes in their peripheral blood at five months post-transplant indicating a myeloid bias similar to responses of aged hematopoietic stem cells (HSC) to an acute inflammatory challenge. We also investigated whether E-cigarette exposure enhances the selective advantage of hematopoietic cells with myeloid malignancy associated mutations. E-cigarette exposure for one month slightly increased mutant cells in peripheral blood but did not have an impact on cells. Altogether, our findings reveal that chronic E-cigarette exposure for two months alters the bone marrow HSPC populations but does not affect HSC reconstitution in primary transplants.
电子烟会产生供人吸入的含尼古丁气溶胶，已成为青少年和年轻成年人中流行的烟草产品，但由于其引入时间相对较近，人们对其健康影响知之甚少。很少有研究评估吸入电子烟烟雾或蒸汽的长期影响。在此，我们表明，两个月的电子烟暴露会导致骨髓造血干细胞和祖细胞（HSPCs）受到抑制。具体而言，与暴露于空气的小鼠相比，暴露于电子烟的动物中常见髓系祖细胞和粒细胞-巨噬细胞祖细胞减少。骨髓移植中的竞争性重建不受两个月电子烟暴露的影响。当使用脂多糖（LPS）对暴露于空气和电子烟的小鼠进行炎症刺激挑战时，两组之间的竞争适应性没有显著差异。然而，移植了来自暴露于电子烟加LPS的小鼠骨髓的小鼠在移植后五个月外周血中的单核细胞升高，表明存在类似于老年造血干细胞（HSC）对急性炎症挑战反应的髓系偏向。我们还研究了电子烟暴露是否会增强具有髓系恶性肿瘤相关突变的造血细胞的选择性优势。一个月的电子烟暴露会使外周血中的突变细胞略有增加，但对细胞没有影响。总之，我们的研究结果表明，两个月的慢性电子烟暴露会改变骨髓HSPC群体，但不会影响初次移植中的HSC重建。
