Microbial Biology, Cellular Biology Department, Biology Institute, University of Brasília, Brasilia, DF, 70919-970, Brazil.
Enzymology Laboratory, Cellular Biology Department, Biology Institute, University of Brasília, Brasilia, DF, 70919-970, Brazil.
Microb Ecol. 2021 Apr;81(3):563-578. doi: 10.1007/s00248-020-01574-x. Epub 2020 Aug 23.
Natural ponds in the Brazilian Cerrado harbor high biodiversity but are still poorly studied, especially their microbial assemblage. The characterization of the microbial community in aquatic environments is fundamental for understanding its functioning, particularly under the increasing pressure posed by land conversion and climate change. Here, we aim to characterize the structure (abundance, richness, and diversity) and composition of the Bacteria and Archaea in the sediment of two natural ponds belonging to different basins that primarily differ in size and depth in the Cerrado. Sediment samples were collected in the dry and rainy seasons and the transition periods between both. The structure and composition of Bacteria and Archaea were assessed by 16S rRNA gene pyrosequencing. We identified 45 bacterial and four archaeal groups. Proteobacteria and Acidobacteria dominated the bacterial community, while Euryarchaeota and Thaumarchaeota dominated the archaeal community. Seasonal fluctuations in the relative abundance of microbial taxa were observed, but pond characteristics were more determinant to community composition differences. Microbial communities are highly diverse, and local variability could partially explain the microbial structure's main differences. Functional predictions based in 16S rRNA gene accessed with Tax4Fun indicated an enriched abundance of predicted methane metabolism in the deeper pond, where higher abundance of methanogenic archaea Methanocella, Methanosaeta, and Methanomicrobiaceae was detected. Our dataset encompasses the more comprehensive survey of prokaryotic microbes in Cerrado's aquatic environments. Here, we present basic and essential information about composition and diversity, for initial insights into the ecology of Bacteria and Archaea in these environments.
巴西塞拉多的自然池塘拥有丰富的生物多样性，但仍未得到充分研究，尤其是其微生物组合。水生环境中微生物群落的特征对于理解其功能至关重要，尤其是在土地转换和气候变化带来的压力不断增加的情况下。在这里，我们旨在描述属于不同流域的两个自然池塘的沉积物中细菌和古菌的结构（丰度、丰富度和多样性）和组成，这两个池塘主要在大小和深度上有所不同。在旱季和雨季以及两者之间的过渡时期采集了沉积物样本。通过 16S rRNA 基因焦磷酸测序评估了细菌和古菌的结构和组成。我们鉴定了 45 个细菌和 4 个古菌群。变形菌门和酸杆菌门是细菌群落的主要优势类群，而广古菌门和奇古菌门是古菌群落的主要优势类群。观察到微生物分类群的相对丰度季节性波动，但池塘特征对群落组成差异的决定作用更大。微生物群落高度多样化，局部变异性可以部分解释微生物结构的主要差异。基于 Tax4Fun 访问的 16S rRNA 基因进行的功能预测表明，在较深的池塘中，预测的甲烷代谢丰度增加，在那里检测到丰度较高的产甲烷古菌 Methanocella、Methanosaeta 和 Methanomicrobiaceae。我们的数据集包含了对塞拉多水生环境中原核微生物的更全面调查。在这里，我们提供了关于组成和多样性的基本和必要信息，为初步了解这些环境中细菌和古菌的生态学提供了依据。
