Department of Operating Room, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, China.
Department of Surgery, The Affiliated Hospital of Qingdao University , Qingdao, Shandong, China.
Bioengineered. 2020 Dec;11(1):939-948. doi: 10.1080/21655979.2020.1814660.
The underlying mechanisms of recurrence and metastasis of epithelial ovarian cancer (EOC) are largely unknown. In the present study, we investigated the clinical significance of microRNA-125b (miR-125b) and its role in ovarian tumorigenesis and progression. Seventy patients of EOC and paired tissues were enrolled from 2015 to 2017. qRT-PCR was used to evaluate miR-125b expression in tumor tissues and EOC cell line. Gain-and-loss function of miR-125b was achieved to explore the changes in cell biological function. We found that miR-125b expression in EOC tissues, especially in the high-grade tissues (P < 0.001), was significantly lower compared to the matched adjacent noncancerous tissues and associated with pathological type, stage, and overall survival (P < 0.05). Upregulation of miR-125b promoted apoptosis and decreased cell survival rate and migration, and vice versa . Mechanistically, miR-125b negatively regulated S100A4, a metastasis-associated protein. MiR-125b overexpression significantly decreased tumor growth and inhibited lung metastasis . Our results supported that miR-125b contributes to the progression of EOC by targeting S100A4. It potentially acts as a potential biomarker and therapeutic target of EOC.
上皮性卵巢癌（EOC）复发和转移的潜在机制在很大程度上尚不清楚。本研究旨在探讨 microRNA-125b（miR-125b）的临床意义及其在卵巢肿瘤发生和进展中的作用。本研究纳入了 2015 年至 2017 年间的 70 名 EOC 患者及其配对组织。qRT-PCR 用于评估肿瘤组织和 EOC 细胞系中 miR-125b 的表达。通过获得和丧失 miR-125b 的功能来探索细胞生物学功能的变化。我们发现，EOC 组织中 miR-125b 的表达，特别是在高级别组织中（P<0.001），明显低于相应的配对非癌组织，并与病理类型、分期和总生存期（P<0.05）相关。miR-125b 的上调促进了细胞凋亡，降低了细胞存活率和迁移率，反之亦然。机制上，miR-125b 负调控 S100A4，一种与转移相关的蛋白。miR-125b 的过表达显著降低了肿瘤生长并抑制了肺转移。我们的研究结果支持 miR-125b 通过靶向 S100A4 促进 EOC 的进展。它可能作为 EOC 的潜在生物标志物和治疗靶点。
