Department of Obstetrics and Gynecology, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, China.
Bioengineered. 2022 Mar;13(3):7034-7048. doi: 10.1080/21655979.2022.2044261.
N6-methyladenosine (m6A) plays a critical role in the tumorigenesis of cervical cancer (CC). Here, we aimed to investigate the potential role of methyltransferase-like 3 (METTL3) in CC. Gene expression was determined via real-time quantitative polymerase chain reaction. Cellular functions were detected using colony formation, 5-ethynyl-2'-deoxyuridine (EdU), and Transwell assays. The interactions among METTL3, insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3), and apoptotic chromatin condensation inducer 1 (ACIN1) were confirmed using the MeRIP and RIP assays. An assay was performed to verify the role of METTL3 in CC development. METTL3 is overexpressed in CC, and therefore, its knockdown inhibits the proliferation and migration of CC cells. Silencing METTL3 inhibits tumor growth . Moreover, a positive association was observed between METTL3 and ACIN1. METTL3 interacts with IGF2BP3 to promote the mRNA stability of ACIN1, the overexpression of which induces the aggressiveness of CC cells. METTL3 promotes ACIN1 mRNA stability to accelerate CC progression, implying that METTL3 is a promising biomarker in CC.
N6-甲基腺苷（m6A）在宫颈癌（CC）的发生发展中起关键作用。在这里，我们旨在研究甲基转移酶样 3（METTL3）在 CC 中的潜在作用。通过实时定量聚合酶链反应测定基因表达。通过集落形成、5-乙炔基-2'-脱氧尿苷（EdU）和 Transwell 测定检测细胞功能。通过 MeRIP 和 RIP 测定证实了 METTL3、胰岛素样生长因子 2 mRNA 结合蛋白 3（IGF2BP3）和凋亡染色质凝聚诱导因子 1（ACIN1）之间的相互作用。进行了一项 试验以验证 METTL3 在 CC 发展中的作用。METTL3 在 CC 中过表达，因此其敲低抑制 CC 细胞的增殖和迁移。沉默 METTL3 抑制肿瘤生长。此外，观察到 METTL3 与 ACIN1 之间存在正相关。METTL3 与 IGF2BP3 相互作用，促进 ACIN1 mRNA 的稳定性，其过表达诱导 CC 细胞的侵袭性。METTL3 促进 ACIN1 mRNA 的稳定性以加速 CC 的进展，这表明 METTL3 是 CC 中有前途的生物标志物。
