Bazghaleh Navid, Prashar Pratibha, Woo Sheridan, Vandenberg Albert
Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK S7N5A8, Canada.
Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy.
Microorganisms. 2020 Aug 24;8(9):1290. doi: 10.3390/microorganisms8091290.
species are opportunistic plant symbionts that are common in the root and rhizosphere ecosystems. Many species may enhance plant growth, nutrient acquisition, and disease resistance, and for these reasons, they are widely used in agriculture as biofertilizers or biocontrol agents. Host plant genotype and other microorganisms, such as root pathogens, may influence the efficacy of inoculants. is an important soil-borne oomycete in western Canada that causes root rot in legume crops such as lentil and pea, and there is not yet any significantly resistant varieties or effective treatments available to control the disease. In this study, the composition of root-associated fungal communities and the abundance of species, strain T-22 and strain G41, was determined in the roots of eight genotypes based on internal transcribed spacer (ITS) Illumina MiSeq paired-end sequencing, both in the presence and the absence of the root rot pathogen . Biocontrol effects of on was also examined. Significant genotypic variations were observed in the composition of root-associated fungal communities and the abundance of the different species in the lentil roots. The presence of altered the composition of found associated to the lentil genotypes. Biocontrol of by T22 species was observed in vitro and positive correlations between the abundance of and plant root and shoot biomass were observed in vivo. These findings revealed that lentil genotype and infection by the phytopathogen greatly influenced the colonization of root-associated fungi and the abundance of the species, as well as the effect on plant growth promotion. The multipartite interactions observed among lentil genotypes, species and suggest possibilities to select compatible host-beneficial microbe combinations in lentil breeding programs and to develop application strategies to harness the beneficial effects of inoculants in sustainable crop production systems.
这些物种是机会主义的植物共生体，在根和根际生态系统中很常见。许多物种可以促进植物生长、养分获取和抗病性，因此，它们作为生物肥料或生物防治剂在农业中被广泛使用。寄主植物基因型和其他微生物，如根病原体，可能会影响接种剂的功效。在加拿大西部，是一种重要的土传卵菌，会导致扁豆和豌豆等豆类作物根腐病，目前还没有任何显著抗性品种或有效的治疗方法来控制这种疾病。在本研究中，基于内转录间隔区（ITS）Illumina MiSeq双端测序，在有和没有根腐病原体的情况下，测定了八种基因型扁豆根中根相关真菌群落的组成以及物种、菌株T-22和菌株G41的丰度。还研究了对的生物防治效果。在扁豆根中，观察到根相关真菌群落组成和不同物种丰度存在显著的基因型差异。的存在改变了与扁豆基因型相关的的组成。在体外观察到T22物种对的生物防治作用，在体内观察到与植物根和地上部生物量之间的正相关。这些发现表明，扁豆基因型和植物病原体的感染极大地影响了根相关真菌的定殖和物种的丰度，以及对植物生长促进的影响。在扁豆基因型、物种和之间观察到的多部分相互作用表明，有可能在扁豆育种计划中选择相容的寄主-有益微生物组合，并制定应用策略，以利用接种剂在可持续作物生产系统中的有益作用。
