Riles L, Olson M V
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
Genetics. 1988 Apr;118(4):601-7. doi: 10.1093/genetics/118.4.601.
A new method for isolating nonsense mutations in essential yeast genes has been used to develop a collection of 115 ochre mutations that define 94 complementation groups. The mutants are isolated in a genetic background that includes an ochre suppressor on a metastable plasmid and a suppressible colony-color marker on a chromosome. When the parental strain is plated on a rich medium, the colonies display a pattern of red, plasmid-free sectors on a white background. Mutants containing an ochre mutation in any essential yeast gene give rise to nonsectoring, white colonies, since cell growth is dependent on the presence of the plasmid-borne suppressor. Analysis of the data suggests that mutations are being recovered from a pool of approximately 250 genes.
一种用于分离酵母必需基因中无义突变的新方法已被用于构建一个包含115个赭石型突变的集合，这些突变定义了94个互补群。这些突变体是在一个遗传背景中分离得到的，该背景包括一个位于不稳定质粒上的赭石型抑制子和一个位于染色体上的可抑制菌落颜色标记。当亲本菌株接种在丰富培养基上时，菌落在白色背景上呈现出红色、无质粒扇形区域的图案。在任何酵母必需基因中含有赭石型突变的突变体产生不分扇形的白色菌落，因为细胞生长依赖于质粒携带的抑制子的存在。数据分析表明，突变是从大约250个基因的库中回收的。
