Yan Youyou, Song Dandan, Zhang Xiaoling, Hui Gang, Wang Junnan
Department of Cardiology, Second Hospital of Jilin University, Changchun, China.
Department of Clinical Laboratory, Second Hospital of Jilin University, Changchun, China.
Front Pharmacol. 2020 Aug 5;11:1155. doi: 10.3389/fphar.2020.01155. eCollection 2020.
Heart failure (HF) is a heterogeneous clinical syndrome with a variety of causes, risk factors, and pathology. Clinically, only brain natriuretic peptide (BNP) or its precursor N-terminus proBNP (NTproBNP) has been validated for HF diagnosis, but they are also affected by other conditions, such as female gender, renal disease, and acute coronary syndromes, and false low levels in the setting of obesity or flash pulmonary edema. In addition, there is no one biomarker which could encompass all heart failure phenotypes. Advances in bioinformatics have provided us with large databases that characterize the complex genetic and epigenetic changes associated with human diseases. The use of data mining strategies on public access databases to identify previously unknown disease markers is an innovative approach to identify potential biomarkers or even new therapeutic targets in complex diseases such as heart failure (HF). In this study, we analyzed the genomic and transcription data of HF peripheral blood mononuclear cell (PBMC) samples obtained from the Gene Expression Omnibus data sets using Omicsbean online database (http://www.omicsbean.cn/) and found that the prostaglandin-endoperoxide synthase 2 (PTGS2), also named as cyclooxygenase-2 (COX-2), as well as its related micro RNAs including miR-1297 and miR-4649-3p might be used as potential biomarkers for non-ischemic heart failure. Our result showed that plasma COX-2 and miR-4649-3p were significantly up-regulated, whereas the plasma miR-1297 was significantly decreased, and miR-4649-3p displayed high predictive power for non-ischemic heart failure.
心力衰竭（HF）是一种具有多种病因、危险因素和病理特征的异质性临床综合征。临床上，仅有脑钠肽（BNP）或其前体N端前脑钠肽（NTproBNP）被证实可用于心力衰竭的诊断，但它们也会受到其他情况的影响，如女性性别、肾脏疾病和急性冠脉综合征，以及肥胖或急性肺水肿时出现的假低水平。此外，没有一种生物标志物能够涵盖所有心力衰竭表型。生物信息学的进展为我们提供了大型数据库，这些数据库描绘了与人类疾病相关的复杂遗传和表观遗传变化。利用数据挖掘策略在公共访问数据库中识别先前未知的疾病标志物，是一种在诸如心力衰竭（HF）等复杂疾病中识别潜在生物标志物甚至新治疗靶点的创新方法。在本研究中，我们使用Omicsbean在线数据库（http://www.omicsbean.cn/）分析了从基因表达综合数据集获得的HF外周血单个核细胞（PBMC）样本的基因组和转录数据，发现前列腺素内过氧化物合酶2（PTGS2），也称为环氧化酶-2（COX-2），以及其相关的微小RNA，包括miR-1297和miR-4649-3p，可能用作非缺血性心力衰竭的潜在生物标志物。我们的结果显示，血浆COX-2和miR-4649-3p显著上调，而血浆miR-1297显著降低，并且miR-4649-3p对非缺血性心力衰竭具有较高的预测能力。
