微小RNA-126通过ERK/MAPK途径下调肌球蛋白轻链激酶来调节棕榈酸酯诱导的人脐静脉内皮细胞迁移。

MicroRNA-126 Modulates Palmitate-Induced Migration in HUVECs by Downregulating Myosin Light Chain Kinase via the ERK/MAPK Pathway.

作者信息

Wang Yi, Wang Mei, Yu Pei, Zuo Li, Zhou Qing, Zhou Xiaomei, Zhu Huaqing

机构信息

Department of Biological Engineering, School of Life Sciences, Anhui Medical University, Hefei, China.

Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei, China.

出版信息

Front Bioeng Biotechnol. 2020 Jul 31;8:913. doi: 10.3389/fbioe.2020.00913. eCollection 2020.

MicroRNA-126 (miR-126) is an endothelial-specific microRNA that has shown beneficial effects on endothelial dysfunction. However, the underlying molecular mechanism is unclear. The present study evaluated the effects of miR-126 on the cell migration and underlying mechanism in HUVECs treated with palmitate. The present results demonstrated that overexpression of miR-126 was found to decrease cell migration in palmitate-treated HUVECs, with decreased MLCK expression and subsequent decreased phosphorylated MLC level. miR-126 also decreased the phosphorylation of MYPT1 in palmitate-treated HUVECs. In addition, it was demonstrated that miR-126 decreases expression of the NADPH oxidase subunits, p67 and Rac family small GTPase 1 with a subsequent decrease in cell apoptosis. Moreover, the phosphorylation of ERK was reduced by miR-126 in palmitate-induced HUVECs. Taken together, the present study showed that the effect of miR-126 on cell migration and cell apoptosis is mediated through downregulation of MLCK via the ERK/MAPK pathway.

微小RNA-126（miR-126）是一种内皮细胞特异性微小RNA，已显示出对内皮功能障碍具有有益作用。然而，其潜在的分子机制尚不清楚。本研究评估了miR-126对棕榈酸处理的人脐静脉内皮细胞（HUVECs）细胞迁移的影响及其潜在机制。目前的结果表明，在棕榈酸处理的HUVECs中，miR-126过表达可降低细胞迁移，同时肌球蛋白轻链激酶（MLCK）表达降低，随后磷酸化的肌球蛋白轻链（ML）水平降低。miR-126还可降低棕榈酸处理的HUVECs中MYPT1的磷酸化水平。此外，已证明miR-126可降低NADPH氧化酶亚基p67和Rac家族小GTP酶1的表达，随后细胞凋亡减少。此外，在棕榈酸诱导的HUVECs中，miR-126可降低细胞外信号调节激酶（ERK）的磷酸化水平。综上所述，本研究表明，miR-126对细胞迁移和细胞凋亡的影响是通过ERK/丝裂原活化蛋白激酶（MAPK）途径下调MLCK介导的。