Department of Oral Implantology, Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, China.
State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing, China.
Microbiol Spectr. 2024 Oct 3;12(10):e0367923. doi: 10.1128/spectrum.03679-23. Epub 2024 Sep 6.
Bacterial biofilms are the major etiology agent of peri-implant disease. Chemical decontamination is a promising treatment strategy against bacterial biofilms; however, its applications are limited by its low efficiency and poor biocompatibility. In contrast to three conventional cleaners (sterile saline, hydrogen peroxide, and chlorhexidine), this study used resveratrol and naringin solutions to remove mature and biofilm on sandblasted (with large grit and acid-etched (SLA) titanium surface. To determine changes in surface characteristics, the surface wettability and roughness were measured, and micromorphology was observed by scanning electron microscopy. With crystal violet (CV) and live/dead bacterial staining, residual plaque quantity and composition were measured. The biocompatibility was tested using pH and cytotoxicity, as well as by osteoblasts (MC3T3-E1) adhesion, proliferation, and differentiation, and fibroblasts (L-929) proliferation were also analyzed. It was found that resveratrol and naringin solutions were more effective in restoring surface characteristics and also showed that less plaque and viable bacteria were left. Naringin removed biofilms better than chlorhexidine. Alkaline resveratrol and naringin solutions increased cell adhesion, proliferation, and osteogenic differentiation without any cytotoxicity. Resveratrol increased the expression of mRNA and protein associated with osteogenesis. In conclusion, resveratrol and naringin effectively restored SLA titanium surface characteristics and decontaminated the biofilm with good biocompatibility, suggesting their therapeutic potential as chemical decontaminants.
Bacterial biofilms are considered the primary etiology of peri-implant disease. Physical cleaning is the most common way to remove bacterial biofilm, but it can cause grooving, melting, and deposition of chemicals that alter the surface of implants, which may hamper biocompatibility and re-osseointegration. Chemical decontamination is one of the most promising treatments but is limited by low efficiency and poor biocompatibility. Our study aims to develop safer, more effective chemical decontaminants for peri-implant disease prevention and treatment. We focus on resveratrol and naringin, two natural compounds, which have shown to be more effective in decontaminating biofilms on dental implant surfaces and exerting better biocompatibility. This research is groundbreaking as it is the first exploration of natural plant extracts' impact on mature bacterial biofilms on rough titanium surfaces. By advancing this knowledge, we seek to contribute to more effective and biocompatible strategies for combating peri-implant diseases, enhancing oral health, and prolonging implant lifespan.
细菌生物膜是种植体周围疾病的主要病因。化学消毒是一种有前途的抗细菌生物膜治疗策略; 然而，其应用受到其低效率和较差的生物相容性的限制。与三种传统清洁剂（无菌盐水、过氧化氢和洗必泰）相比，本研究使用白藜芦醇和柚皮苷溶液去除喷砂（大粒度和酸蚀（SLA）钛表面上的成熟和生物膜。为了确定表面特性的变化，测量了表面润湿性和粗糙度，并通过扫描电子显微镜观察了微观形态。使用结晶紫（CV）和活菌/死菌染色，测量残留菌斑的数量和组成。通过 pH 值和细胞毒性测试以及成骨细胞（MC3T3-E1）的粘附、增殖和分化来测试生物相容性，还分析了成纤维细胞（L-929）的增殖。结果发现，白藜芦醇和柚皮苷溶液在恢复表面特性方面更有效，并且表明留下的菌斑和活菌更少。柚皮苷比洗必泰更能去除生物膜。碱性白藜芦醇和柚皮苷溶液增加了细胞粘附、增殖和成骨分化，而没有任何细胞毒性。白藜芦醇增加了与成骨相关的 mRNA 和蛋白的表达。总之，白藜芦醇和柚皮苷有效地恢复了 SLA 钛表面特性，并具有良好的生物相容性去除生物膜，表明它们作为化学消毒剂具有治疗潜力。
细菌生物膜被认为是种植体周围疾病的主要病因。物理清洁是去除细菌生物膜的最常见方法，但它会导致沟槽、融化和化学物质的沉积，从而改变植入物的表面，这可能会妨碍生物相容性和再骨整合。化学消毒是最有前途的治疗方法之一，但受到效率低和生物相容性差的限制。我们的研究旨在开发更安全、更有效的化学消毒剂，用于预防和治疗种植体周围疾病。我们专注于白藜芦醇和柚皮苷，这两种天然化合物已被证明在去除牙种植体表面生物膜方面更有效，并具有更好的生物相容性。这项研究是开创性的，因为它是首次探索天然植物提取物对粗糙钛表面成熟细菌生物膜的影响。通过推进这方面的知识，我们旨在为更有效的和生物相容的策略做出贡献，以对抗种植体周围疾病，提高口腔健康，并延长植入物的寿命。
