Badaracco Adrian Garcia, Ward Erin, Barback Christopher, Yang Jian, Wang James, Huang Ching-Hsin, Kim Moon, Wang Qingxiao, Nam Seungjin, Delong Jonathan, Blair Sarah, Trogler William C, Kummel Andrew
Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
Department of Surgery, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
Appl Surf Sci. 2020 Jan 1;499. doi: 10.1016/j.apsusc.2019.143885. Epub 2019 Sep 5.
Marking colon tumors for surgery is normally done with the use of India ink. However, non-fluorescent dyes such as India ink cannot be imaged below the tissue surface and there is evidence for physiological complications such as abscess, intestinal perforation and inconsistency of dye injection. A novel infrared marker was developed using FDA approved indocyanine green (ICG) dye and ultrathin hollow silica nanoshells (ICG/HSS). Using a positively charged amine linker, ICG was non-covalently adsorbed onto the nanoparticle surface. For ultra-thin wall 100 nm diameter silica shells, a bimodal ICG layer of < 3 nm is was formed. Conversely, for thicker walls on 2 μm diameter silica shells, the ICG layer was only bound to the outer surface and was 6 nm thick. In vitro testing of fluorescent emission showed the particles with the thinner coating were considerably more efficient, which is consistent with self-quenching reducing emission shown in the thicker ICG coatings. Ex-vivo testing showed that ICG bound to the 100 nm hollow silica shells was visible even under 1.5 cm of tissue. In vivo experiments showed that there was no diffusion of the ICG/nanoparticle marker in tissue and it remained imageable for as long as 12 days.
标记结肠肿瘤以便手术通常使用印度墨水。然而，诸如印度墨水之类的非荧光染料无法在组织表面以下成像，并且有证据表明存在诸如脓肿、肠穿孔和染料注射不一致等生理并发症。一种新型红外标记物是使用美国食品药品监督管理局（FDA）批准的吲哚菁绿（ICG）染料和超薄中空二氧化硅纳米壳（ICG/HSS）开发的。通过带正电荷的胺连接体，ICG非共价吸附在纳米颗粒表面。对于直径100nm的超薄壁二氧化硅壳，形成了厚度小于3nm的双峰ICG层。相反，对于直径2μm的较厚壁二氧化硅壳，ICG层仅结合在外表面，厚度为6nm。荧光发射的体外测试表明，涂层较薄的颗粒效率要高得多，这与较厚ICG涂层中显示的自猝灭降低发射一致。离体测试表明，即使在1.5cm厚的组织下，与100nm中空二氧化硅壳结合的ICG也是可见的。体内实验表明，ICG/纳米颗粒标记物在组织中不会扩散，并且在长达12天的时间内都可成像。
