喀麦隆西部肠道感染成年患者粪便分离株中肠道病毒的毒力和抗菌药物耐药性标志物分析
Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon.
作者信息
Marbou Wiliane J T, Jain Priyanka, Samajpati Sriparna, Halder Gourab, Mukhopadhyay Asish K, Dutta Shanta, Kuete Victor
机构信息
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
Bacteriology Division, Indian Council of Medical Research-National Institute of Cholera and Enteric Diseases, Kolkata, India.
出版信息
Osong Public Health Res Perspect. 2020 Aug;11(4):216-230. doi: 10.24171/j.phrp.2020.11.4.11.
OBJECTIVES
This study aimed to identify virulent and antimicrobial resistant genes in fecal in Mbouda, Cameroon.
METHODS
A total of 599 fecal samples were collected from patients with enteric infections who were ≥ 20 years old. was isolated on the MacConkey agar and virulent genes were detected by multiplex/simplex PCR. Isolates in which ≥ 1 virulent gene was detected were subjected to antibiotic susceptibility testing. The resulting resistant isolates were subjected to PCR, followed by sequencing for resistant genes detection.
RESULTS
There were 119 enterovirulent identified, amongst which 47.05% were atypical enteropathogenic (EPEC), 36.97% enterotoxigenic , 10.08% Shiga toxin producing (STEC) and 5.88% were enteroinvasive (EIEC). The occurrence of the gene (47.06%) was higher compared with (33.61%), (13.45%), (10.08%) and (0.84%). High resistance rates were noted for ampicillin (94.64% EPEC, 91.67% STEC, 59.09% EAEC, and 57.14% EIEC) and sulfamethoxazole-trimethoprim (100% EPEC and 83.33% STEC, 81.82% EAEC and 71.43% EIEC). (71.43%), (64.71%), (59.94%) and (52.10%) were detected. A double mutation (S83L; D87N) was seen in and a single mutation (S80I) was observed in .
CONCLUSION
These findings suggested that measures should be taken to reduce the harm of to public health.
目的
本研究旨在鉴定喀麦隆姆布达地区粪便中的致病基因和抗菌耐药基因。
方法
从年龄≥20岁的肠道感染患者中总共收集了599份粪便样本。在麦康凯琼脂上分离菌株，并通过多重/单重聚合酶链反应（PCR）检测致病基因。对检测到≥1个致病基因的分离株进行药敏试验。对产生耐药性的分离株进行PCR，随后进行测序以检测耐药基因。
结果
共鉴定出119株肠道致病菌株，其中47.05%为非典型肠致病性大肠杆菌（EPEC），36.97%为产肠毒素大肠杆菌，10.08%为产志贺毒素大肠杆菌（STEC），5.88%为侵袭性大肠杆菌（EIEC）。肠毒素基因（47.06%）的出现率高于热稳定毒素基因（33.61%）、耐热肠毒素基因（13.45%）、志贺毒素基因（10.08%）和侵袭性质粒抗原基因（0.84%）。氨苄西林（EPEC为94.64%、STEC为91.67%、EAEC为59.09%、EIEC为57.14%）和复方新诺明（EPEC为100%、STEC为83.33%、EAEC为81.82%、EIEC为71.43%）的耐药率较高。检测到超广谱β-内酰胺酶（71.43%）、头孢菌素酶（64.71%）、喹诺酮耐药决定区（59.94%）和氨基糖苷类修饰酶（52.10%）。在超广谱β-内酰胺酶中发现双突变（S83L；D87N），在喹诺酮耐药决定区中观察到单突变（S80I）。
结论
这些发现表明应采取措施减少大肠杆菌对公众健康的危害。
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