Jiang Qingru, Kainulainen Veera, Stamatova Iva, Janket Sok-Ja, Meurman Jukka H, Korpela Riitta
Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, 00100 Helsinki, Finland.
Department of Pharmacology, Faculty of Medicine, University of Helsinki, 00100 Helsinki, Finland.
Dent J (Basel). 2020 Sep 1;8(3):96. doi: 10.3390/dj8030096.
In order to investigate the effects of mouthwashes on oral biofilms with probiotics, we compared in biofilms the susceptibility to mouthwashes of probiotic GG (LGG) and oral pathogens , , and . We also evaluated these pathogens' susceptibility to the mouthwashes and their recovery after mouthwash-rinsing in biofilms with/without LGG. First, 1-day-/3-day-old LGG-integrated multi-species biofilms were exposed for 1 min to mouthwashes containing chlorhexidine, essential oils, or amine fluoride/stannous fluoride. Cells were plate-counted and relative survival rates (RSRs) of LGG and pathogens calculated. Second, 1-day-/3-day-old multispecies biofilms with and without LGG were exposed for 1 min to mouthwashes; cells were plate-counted and the pathogens' RSRs were calculated. Third, 1-day-old biofilms were treated for 1 min with mouthwashes. Cells were plate-counted immediately and after 2-day cultivation. Recovery rates of pathogens were calculated and compared between biofilms with/without LGG. Live/Dead staining served for structural analyses. Our results showed that RSRs of LGG were insignificantly smaller than those of pathogens in both 1-day and 3-day biofilms. No significant differences appeared in pathogens' RSRs and recovery rates after treatment between biofilms with/without LGG. To conclude, biofilm LGG was susceptible to the mouthwashes; but biofilm LGG altered neither the mouthwash effects on oral pathogens nor affected their recovery.
为了研究含益生菌的漱口水对口腔生物膜的影响，我们在生物膜中比较了益生菌GG（LGG）和口腔病原体、、和对漱口水的敏感性。我们还评估了这些病原体对漱口水的敏感性以及在含/不含LGG的生物膜中漱口水冲洗后它们的恢复情况。首先，将1日龄/3日龄整合LGG的多物种生物膜暴露于含氯己定、精油或胺氟化物/氟化亚锡的漱口水1分钟。对细胞进行平板计数并计算LGG和病原体的相对存活率（RSR）。其次，将含和不含LGG的1日龄/3日龄多物种生物膜暴露于漱口水1分钟；对细胞进行平板计数并计算病原体的RSR。第三，用漱口水处理1日龄生物膜1分钟。立即和培养2天后对细胞进行平板计数。计算病原体的恢复率并比较含/不含LGG的生物膜之间的差异。采用活/死染色进行结构分析。我们的结果表明，在1日龄和3日龄生物膜中，LGG的RSR均略小于病原体的RSR。含/不含LGG的生物膜在处理后病原体的RSR和恢复率没有显著差异。总之，生物膜LGG对漱口水敏感；但生物膜LGG既不改变漱口水对口腔病原体的作用，也不影响它们的恢复。
