Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Mar;72(3). doi: 10.1099/ijsem.0.005272.
A Gram-negative, moderately halophilic bacterium, designated as strain Y3S6, was isolated from a surface seawater sample collected from Dongangyoeng cave, Udo-myeon, Jeju-si, Jeju-do, Repulic of Korea. Cells of strain Y3S6 were aerobic, rod-shaped, non-sporulated, yellow, catalase- negative, oxidase-negative and motile with one polar flagellum. Growth of strain Y3S6 occurred at 15-40 °C (optimum: 25-30 °C), at pH 6.0-9.0 (optimum: pH 7.0) and in the presence of 0-13% NaCl (optimum: 1-6 %, w/v). The novel strain was able to produce carotenoids. Its chemotaxonomic and morphological characteristics were consistent with those of members of the genus . Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Y3S6 formed a clade with L5 (98.97 %) and LCB169(98.90%). The average nucleotide identity and digital DNA-DNA hybridization values of strain Y3S6 with the most closely related strains for which whole genomes are publicly available were 82.3-85.2% and 62.8-66.1 %, respectively. The major fatty acids in strain Y3S6 were C, C cyclo ω8 and summed feature 8 (composed of C ω7 and/or C ω6), and the predominant quinone was Q-9. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phosphoglycolipid, one unidentified phosphoaminoglycolipid and one unidentified phospholipid. The genomic DNA G+C content based on the draft genome sequence was 64.2 mol%. The results of physiological and biochemical tests and 16S rRNA sequence analysis clearly revealed that strain Y3S6 represents a novel species in the genus , for which the name sp. nov. has been proposed. The type strain is Y3S6 (=KACC 21536=NBRC 114315=TBRC 15164).
一株革兰氏阴性、中度嗜盐的细菌，被命名为 Y3S6 菌株，是从韩国济州道牛岛面东洋英洞的表层海水中分离出来的。Y3S6 菌株的细胞为需氧、杆状、非孢子形成、黄色、过氧化氢酶阴性、氧化酶阴性且具有一根极生鞭毛，可运动。Y3S6 菌株的生长温度为 15-40°C（最佳温度：25-30°C），pH 值为 6.0-9.0（最佳 pH 值：7.0），盐度为 0-13%（最佳盐度：1-6%，w/v）。该新菌株能够产生类胡萝卜素。其化学分类学和形态特征与属的成员一致。16S rRNA 基因序列的系统发育分析表明，菌株 Y3S6 与 L5（98.97%）和 LCB169（98.90%）形成一个分支。与具有完整基因组的最密切相关的菌株相比，菌株 Y3S6 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 82.3-85.2%和 62.8-66.1%。菌株 Y3S6 的主要脂肪酸为 C 、 C 环 ω8 和总和特征 8（由 C ω7 和/或 C ω6 组成），主要醌为 Q-9。其极性脂谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷酸甘油糖脂、一种未鉴定的磷酸氨基甘油糖脂和一种未鉴定的磷脂组成。基于基因组草图序列的基因组 DNA G+C 含量为 64.2mol%。生理生化试验和 16S rRNA 序列分析的结果清楚地表明，菌株 Y3S6 代表了属中的一个新种，建议将其命名为 sp. nov.。模式菌株为 Y3S6（=KACC 21536=NBRC 114315=TBRC 15164）。
