Russell Grace, Zulfiqar Faisal, Hancock John T
Department of Applied Sciences, University of the West of England, Bristol BS 16 1QY, UK.
Institute of Horticultural Sciences, Faculty of Agriculture, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan.
Plants (Basel). 2020 Sep 2;9(9):1136. doi: 10.3390/plants9091136.
Molecular hydrogen (H) has been suggested to be a beneficial treatment for a range of species, from humans to plants. Hydrogenases catalyze the reversible oxidation of H, and are found in many organisms, including plants. One of the cellular effects of H is the selective removal of reactive oxygen species (ROS) and reactive nitrogen species (RNS), specifically hydroxyl radicals and peroxynitrite. Therefore, the function of hydrogenases and the action of H needs to be reviewed in the context of the signalling roles of a range of redox active compounds. Enzymes can be controlled by the covalent modification of thiol groups, and although motifs targeted by nitric oxide (NO) can be predicted in hydrogenases sequences it is likely that the metal prosthetic groups are the target of inhibition. Here, a selection of hydrogenases, and the possibility of their control by molecules involved in redox signalling are investigated using a bioinformatics approach. Methods of treating plants with H along with the role of H in plants is also briefly reviewed. It is clear that studies report significant effects of H on plants, improving growth and stress responses, and therefore future work needs to focus on the molecular mechanisms involved.
分子氢（H₂）已被认为是对从人类到植物等一系列物种的有益治疗手段。氢化酶催化H₂的可逆氧化反应，并且存在于包括植物在内的许多生物体中。H₂的细胞效应之一是选择性清除活性氧（ROS）和活性氮（RNS），特别是羟基自由基和过氧亚硝酸盐。因此，需要在一系列氧化还原活性化合物的信号传导作用背景下，对氢化酶的功能和H₂的作用进行综述。酶可以通过硫醇基团的共价修饰来控制，尽管在氢化酶序列中可以预测一氧化氮（NO）靶向的基序，但金属辅基很可能是抑制作用的靶点。在这里，我们使用生物信息学方法研究了一系列氢化酶，以及它们被参与氧化还原信号传导的分子控制的可能性。同时也简要综述了用H₂处理植物的方法以及H₂在植物中的作用。很明显，研究报告了H₂对植物有显著影响，可改善生长和应激反应，因此未来的工作需要关注其中涉及的分子机制。
