Protects against LPS-Induced Colonic Inflammation via IL-22 Signaling in Mice.

This study aimed to investigate the effects of on colonic immunity and the role of IL-22 in the protective function of . Mice were supplemented with 10 CFU/mL daily for 20 days, followed by injecting with LPS for 24 h. In vivo interference of IL-22 via injecting with an adeno-associated virus was conducted to elucidate the role of IL-22 in attenuating colonic inflammation. The results showed that decreased the mRNA expression of IL-6 and IL-1β. enhanced the mRNA expression of IL-22 and the expression of MUC2 in the colon. The in vivo interference results showed that enhanced the mRNA expression of IL-6 and IL-1β while decreased the expression of MUC2 after knocking down IL-22. The flow cytometric analysis showed that decreased the proportions of macrophages, DCs, and mast cells and effectively regulated the proportion of Th17 cells, indicating that may stimulate the expression of IL-22 via regulating Th17 cells. Our study concluded that protected against LPS-induced colonic barrier dysfunction and inflammation via IL-22 signaling, suggesting that could be a potential probiotic in regulating colonic health.