Yang Qingxin, Yuan Wen, Zhao Tinghui, Jiao Yanixao, Tang Menghuan, Cong Zhaoqing, Wu Song
Department of Pharmacy, Mianyang Orthopaedic Hospital, Mianyang, 621000, People's Republic of China.
Mianyang Key Laboratory of Development and Utilization of Chinese Medicine Resources, Mianyang, 621000, People's Republic of China.
Drug Des Devel Ther. 2024 Dec 7;18:5841-5851. doi: 10.2147/DDDT.S490652. eCollection 2024.
Bladder cancer has high recurrence rates despite standard treatments, necessitating innovative therapeutic approaches. This study introduces magnetically powered microrobots utilizing Traditional Chinese Medicine (TCM) Spora Lygodii (SL) encapsulated with Doxorubicin (DOX) and FeO nanoparticles (Fe/DOX@SL) for targeted therapy.
FeO nanoparticles were synthesized via co-precipitation and combined with SL spores and DOX through dip-coating to form Fe/DOX@SL microrobots. Their propulsion was controlled by a rotating magnetic field (RMF) for precise delivery. The microrobots' mobility and adherence were assessed in various biological media. Therapeutic efficacy was evaluated using an orthotopic bladder cancer model in mice treated intravesically with Fe/DOX@SL under RMF guidance, compared to controls.
Fe/DOX@SL microrobots demonstrated efficient movement and stable navigation in biological environments. In vivo experiments showed superior retention in the bladder, prolonged adherence to the mucosa, and significantly enhanced tumor suppression in the RMF-guided group. Bioluminescence imaging confirmed reduced tumor growth, and histological analysis revealed substantial tumor regression compared to other treatments.
This study highlights the potential of integrating TCM with advanced microrobotics. The biocompatible Fe/DOX@SL microrobots leverage SL's therapeutic properties and fuel-free magnetic control to overcome challenges in bladder cancer treatment, such as poor drug retention and off-target toxicity. This novel platform represents a promising advancement in targeted cancer therapy. The innovative fusion of TCM and microrobotics introduces a potent, targeted therapeutic strategy for bladder cancer, paving the way for broader biomedical applications.
尽管采用了标准治疗方法，膀胱癌的复发率仍然很高，因此需要创新的治疗方法。本研究引入了一种磁驱动微型机器人，其利用包裹有阿霉素（DOX）和FeO纳米颗粒（Fe/DOX@SL）的中药海金沙（SL）进行靶向治疗。
通过共沉淀法合成FeO纳米颗粒，并通过浸涂将其与SL孢子和DOX结合，形成Fe/DOX@SL微型机器人。通过旋转磁场（RMF）控制其推进，以实现精确递送。在各种生物介质中评估微型机器人的移动性和粘附性。与对照组相比，在RMF引导下膀胱内注射Fe/DOX@SL治疗小鼠原位膀胱癌模型，评估治疗效果。
Fe/DOX@SL微型机器人在生物环境中表现出高效的移动和稳定的导航。体内实验表明，在RMF引导组中，其在膀胱中的保留效果更好，对黏膜的粘附时间延长，肿瘤抑制作用显著增强。生物发光成像证实肿瘤生长减缓，组织学分析显示与其他治疗方法相比肿瘤有明显消退。
本研究突出了将中药与先进的微型机器人技术相结合的潜力。生物相容性良好的Fe/DOX@SL微型机器人利用SL的治疗特性和无燃料磁控技术，克服了膀胱癌治疗中的挑战，如药物保留不佳和脱靶毒性。这个新平台代表了靶向癌症治疗方面有前景的进展。中药与微型机器人技术的创新融合为膀胱癌引入了一种有效、靶向的治疗策略，为更广泛的生物医学应用铺平了道路。
