Almeida Adriana Luisa Goncalves, Freitas Gileade Pereira, Lopes Helena Bacha, Gimenes Rossano, Siessere Selma, Sousa Luiz Gustavo, Beloti Marcio Mateus, Rosa Adalberto Luiz
Universidade de São Paulo - USP, School of Dentistry of Ribeirão Preto , Bone Research Lab , Ribeirão Preto , SP , Brazil .
Universidade Federal de Itajubá - Unifei, Institute of Physics and Chemistry , Itajubá , MG , Brazil.
Braz Oral Res. 2019 Sep 9;33:e079. doi: 10.1590/1807-3107BOR-2019.vol33.0079.
Cell therapy associated with guided bone regeneration (GBR) can be used to treat bone defects under challenging conditions such as osteoporosis. This study aimed to evaluate the effect of mesenchymal stem cells (MSCs) in combination with a poly(vinylidene-trifluoroethylene)/barium titanate (PVDF-TrFE/BT) membrane on bone repair in osteoporotic rats. Osteoporosis was induced in female rats by bilateral removal of the ovaries (OVX) or sham surgery (SHAM), and the osteoporotic condition was characterized after 5 months by microtomographic and morphometric analyses. Calvarial defects were created in osteoporotic rats that immediately received the PVDF-TrFE/BT membrane. After 2 weeks, bone marrow-derived MSCs from healthy rats, characterized by the expression of surface markers using flow cytometry, or phosphate-buffered saline (PBS) (Control) were injected into the defects and bone formation was evaluated 4 weeks post-injection by microtomographic, morphometric, and histological analyses. A reduction in the amount of bone tissue in the femurs of OVX compared with SHAM rats confirmed the osteoporotic condition of the experimental model. More bone formation was observed when the defects were injected with MSCs compared to that with PBS. The modification that we are proposing in this study for the classical GBR approach where cells are locally injected after a membrane implantation may be a promising therapeutic strategy to increase bone formation under osteoporotic condition.
与引导骨再生（GBR）相关的细胞疗法可用于治疗骨质疏松等具有挑战性条件下的骨缺损。本研究旨在评估间充质干细胞（MSCs）与聚（偏二氟乙烯 - 三氟乙烯）/钛酸钡（PVDF-TrFE/BT）膜联合使用对骨质疏松大鼠骨修复的影响。通过双侧卵巢切除（OVX）或假手术（SHAM）诱导雌性大鼠骨质疏松，5个月后通过显微断层扫描和形态计量分析对骨质疏松状况进行表征。在骨质疏松大鼠中制造颅骨缺损，并立即植入PVDF-TrFE/BT膜。2周后，将通过流式细胞术检测表面标志物表达来表征的健康大鼠骨髓来源的MSCs或磷酸盐缓冲盐水（PBS）（对照）注入缺损处，并在注射后4周通过显微断层扫描、形态计量和组织学分析评估骨形成情况。与SHAM大鼠相比，OVX大鼠股骨中的骨组织量减少证实了实验模型的骨质疏松状况。与注射PBS相比，当缺损处注射MSCs时观察到更多的骨形成。我们在本研究中对经典GBR方法提出的改进，即在植入膜后局部注射细胞，可能是一种在骨质疏松条件下增加骨形成的有前景的治疗策略。
