1Institute for Veterinary Medical Research, Centre for Agricultural Research, P.O. Box 18, Budapest, H-1581 Hungary.
2Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Hungary.
Acta Vet Hung. 2020 Sep 4;68(2):140-146. doi: 10.1556/004.2020.00021.
In this paper we report the phenotypic and partial genetic characterisation of a novel bacterium strain isolated from a cat with severe nephritis. Multilocus sequence analysis was performed on the 16S rRNA and three housekeeping (recN, rpoB, infB) gene sequences obtained by PCR. In accordance with the results of phenotypic tests, the phylogenetic analyses confirmed the relatedness of the new strain (6036) to the family Pasteurellaceae. On the phylogenetic trees, strain 6036 appeared in a separate branch, closest to that of the type species (Frederiksenia canicola) of the genus Frederiksenia. These two bacteria shared 95.14 and 76.88% identity in their partial 16S rRNA and recN gene sequences, respectively. The rpoB- and infB-based phylogenetic analyses indicated that strain 6036 is most closely related to Bibersteinia trehalosi (with 90.58% identity) and [Haemophilus] felis ATCC 49733 (89.50% identity), respectively. The predicted genome identity values, based on the recN gene sequences, suggested that strain 6036 can be classified into the genus Frederiksenia as a novel species. A PCR method, specific to strain 6036, was developed to allow its rapid and accurate identification and differentiation from F. canicola and other species of Pasteurellaceae. The minimal inhibitory concentrations of 18 antimicrobial agents for strain 6036 were also determined.
本文报告了从一只患有严重肾炎的猫身上分离出的一种新型细菌菌株的表型和部分遗传特征。通过 PCR 获得 16S rRNA 和三个管家基因（recN、rpoB、infB）序列，对其进行了多基因序列分析。根据表型试验结果，系统发育分析证实了新菌株（6036）与巴斯德氏菌科的亲缘关系。在系统发育树上，菌株 6036 出现在一个单独的分支中，与弗雷德里克森菌属的模式种（弗雷德里克森氏菌坎icola）最为接近。这两个细菌在部分 16S rRNA 和 recN 基因序列上分别具有 95.14%和 76.88%的同一性。rpoB 和 infB 系统发育分析表明，菌株 6036 与比伯斯坦氏菌（Trehalosi）（同源性为 90.58%）和 [嗜血杆菌] 猫 ATCC 49733（同源性为 89.50%）最为密切相关。基于 recN 基因序列预测的基因组同源性值表明，菌株 6036 可归类为弗雷德里克森氏菌属的一个新种。开发了一种针对菌株 6036 的 PCR 方法，可用于快速准确地鉴定和区分其与 F. canicola 和其他巴斯德氏菌科的物种。还确定了菌株 6036 对 18 种抗菌剂的最小抑菌浓度。
