Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, IL, 61801, USA.
Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Adv Healthc Mater. 2021 Feb;10(4):e2001040. doi: 10.1002/adhm.202001040. Epub 2020 Sep 9.
The field of 3D printing is an area of active research, with a substantial focus given to the design and construction of customized tools for applications in technology. There exists a particular need in these developing areas of opportunity for new multi-functional soft materials that are biologically compatible for the growth and directed culturing of cells. Herein, a composite material consisting of gold nanoparticles with useful plasmonic properties embedded within a highly hydrophilic poly-2-hydroxyethylmethacrylate matrix is described and characterized. This composite material serves dual functions as both host framework scaffold for cell lines such as pre-osteoblasts as well as a plasmonic biosensor for in situ measurements of living cells. The plasmonic properties of this system are characterized as a function of the material properties and related to compositional features of the material through a proposed light-directed mechanism. This chemistry provides a tunable, 3D printable plasmonic composite material of encapsulated gold nanoparticles in a biologically-compliant, acrylate-based hydrogel matrix. Surface-enhanced Raman scattering studies of 3D-microcultures supported by the scaffolds are carried out and the strong influence of perm-selective molecular diffusion in its analytical responses is established. Most notably, specific, largely hydrophilic, cellular metabolites are detected within the supported live cultures.
3D 打印领域是一个活跃的研究领域，其重点主要集中在设计和构建用于技术应用的定制工具上。在这些不断发展的机会领域中，特别需要新的多功能软材料，这些材料需要具有生物相容性，以促进细胞的生长和定向培养。本文描述并表征了一种由金纳米粒子组成的复合材料，这些纳米粒子具有有用的等离子体特性，嵌入在高度亲水性的聚 2-羟乙基甲基丙烯酸酯基质中。这种复合材料具有双重功能，既是前成骨细胞等细胞系的宿主框架支架，也是用于活细胞原位测量的等离子体生物传感器。该系统的等离子体特性作为材料特性的函数进行了表征，并通过提出的光导向机制与材料的组成特征相关联。这种化学方法提供了一种可调节的、3D 可打印的等离子体复合纳米材料，将金纳米粒子封装在生物相容的丙烯酰胺基水凝胶基质中。通过支架支撑的 3D 微培养物进行了表面增强拉曼散射研究，确定了其分析响应中选择性分子扩散的强烈影响。值得注意的是，在支持的活培养物中检测到了特定的、主要亲水的细胞代谢物。
