Dos Santos Cruz Bruna Cristina, da Silva Duarte Vinícius, Giacomini Alessio, Corich Viviana, de Paula Sérgio Oliveira, da Silva Fialho Lilian, Guimarães Valéria Monteze, de Luces Fortes Ferreira Célia Lúcia, Gouveia Peluzio Maria do Carmo
Nutritional Biochemistry Laboratory, Department of Nutrition and Health, Universidade Federal de Viçosa, Avenida P. H. Rolfs, Campus Universitário S/N, Viçosa, Minas Gerais, CEP: 36570-900, Brazil.
Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Legnaro, Italy.
Appl Microbiol Biotechnol. 2020 Oct;104(20):8837-8857. doi: 10.1007/s00253-020-10863-x. Epub 2020 Sep 9.
Colorectal cancer is a public health problem, with dysbiosis being one of the risk factors due to its role in intestinal inflammation. Probiotics and synbiotics have been used in order to restore the microbiota balance and to prevent colorectal carcinogenesis. We aimed to investigate the effects of the probiotic VSL#3® alone or in combination with a yacon-based prebiotic concentrate on the microbiota modulation and its influence on colorectal carcinogenesis in an animal model. C57BL/6J mice were divided into three groups: control (control diet), probiotic (control diet + VSL#3®), and synbiotic (yacon diet + VSL#3®). The diets were provided for 13 weeks and, from the third one, all animals were subjected to induction of colorectal cancer precursor lesions. Stool samples were collected to evaluate organic acids, feces pH, β-glucuronidase activity, and microbiota composition. The colon was used to count pre-neoplastic lesions and to determine the cytokines. The microbiota composition was influenced by the use of probiotic and synbiotic. Modifications were also observed in the abundance of bacterial genera with respect to the control group, which confirms the interference of carcinogenesis in the microbiota. Pre-neoplastic lesions were reduced by the use of the synbiotic, but not with the probiotic. The protection provided by the synbiotic can be attributed to the modulation of the intestinal inflammatory response, to the inhibition of a pro-carcinogenic enzyme, and to the production of organic acids. The modulation of the composition and activity of the microbiota contributed to beneficial changes in the intestinal microenvironment, which led to a reduction in carcinogenesis. KEY POINTS: • Synbiotic reduces the incidence of colorectal cancer precursor lesions. • Synbiotic modulates the composition and activity of intestinal microbiota. • Synbiotic increases the abundance of butyrate-producing bacteria.
结直肠癌是一个公共卫生问题，肠道菌群失调因其在肠道炎症中的作用而成为风险因素之一。益生菌和合生元已被用于恢复微生物群平衡并预防结直肠癌的发生。我们旨在研究单独使用益生菌VSL#3®或与基于雪莲果的益生元浓缩物联合使用对动物模型中微生物群调节的影响及其对结直肠癌发生的影响。C57BL/6J小鼠分为三组：对照组（对照饮食）、益生菌组（对照饮食 + VSL#3®）和合生元组（雪莲果饮食 + VSL#3®）。给予这些饮食13周，从第三周开始，所有动物都接受结直肠癌前体病变的诱导。收集粪便样本以评估有机酸、粪便pH值、β-葡萄糖醛酸酶活性和微生物群组成。使用结肠来计数癌前病变并确定细胞因子。微生物群组成受到益生菌和合生元使用的影响。与对照组相比，细菌属的丰度也观察到了变化，这证实了致癌作用对微生物群的干扰。合生元的使用减少了癌前病变，但益生菌组没有。合生元提供的保护可归因于肠道炎症反应的调节、致癌酶的抑制以及有机酸的产生。微生物群组成和活性的调节有助于肠道微环境的有益变化，从而导致癌症发生率降低。关键点：• 合生元降低了结直肠癌前体病变 的发生率。• 合生元调节肠道微生物群的组成和活性。• 合生元增加了产丁酸细菌的丰度。
