A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Arbuzov Str. 8, 420088 Kazan, Russia.
A.M. Butlerov Chemical Institute, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Russia.
Int J Mol Sci. 2023 Sep 21;24(18):14372. doi: 10.3390/ijms241814372.
Among the plants that exhibit significant or established pharmacological activity, the genus L. deserves special attention. This genus comprises over 500 species belonging to the largest family. Our study aimed at providing a comprehensive evaluation of the phytochemical composition of the ethanol extracts of five different L. species (collected from the southwest of the Russian Federation) and their antimicrobial and nematocide activity as follows: cv. Novichok., cv. Smaragd, cv. Citral, cv. Euxin, and cv. Tavrida. The study of the ethanol extracts of the five different L. species using the methods of gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-MS/MS) allowed establishing their phytochemical profile. The obtained data on the of five different L. species ethanol extracts' phytochemical composition were used to predict the antibacterial and antifungal activity against phytopathogenic microorganisms and nematocidal activity against the free-living soil nematode . The major compounds found in the composition of the L. ethanol extracts were monoterpenes, sesquiterpenes, flavonoids, flavonoid glycosides, coumarins, and phenolic acids. The antibacterial and antifungal activity of the extracts began to manifest at a concentration of 150 µg/mL. The cv. Smaragd extract had a selective effect against Gram-positive and bacteria, whereas the cv. Tavrida extract had a selective effect against Gram-negative and bacteria and , and fungi. The cv. Novichok, cv. Smaragd, and cv. Citral extracts in the concentration range of 31.3-1000 µg/mL caused the death of nematodes. It was established that cv. Novichok affects the UNC-63 protein, the molecular target of which is the nicotine receptor of the N-subtype.
在表现出显著或既定药理活性的植物中，属 L. 值得特别关注。该属包含超过 500 种属于最大科的物种。我们的研究旨在全面评估五种不同的 L. 种（从俄罗斯西南部采集）的乙醇提取物的植物化学成分及其抗菌和杀线虫活性，如下所示： cv. Novichok.，cv. Smaragd，cv. Citral，cv. Euxin 和 cv. Tavrida。使用气相色谱-质谱联用 (GC-MS) 和高效液相色谱-四极杆飞行时间质谱联用 (HPLC-MS/MS) 方法研究五种不同的 L. 种的乙醇提取物，确定了它们的植物化学成分特征。获得的关于五种不同的 L. 种乙醇提取物的植物化学成分的数据用于预测对植物病原菌微生物的抗菌和抗真菌活性以及对自由生活土壤线虫的杀线虫活性。在 L. 乙醇提取物的组成中发现的主要化合物是单萜、倍半萜、类黄酮、类黄酮糖苷、香豆素和酚酸。提取物的抗菌和抗真菌活性在 150 µg/mL 的浓度下开始表现出来。cv. Smaragd 提取物对革兰氏阳性 和 细菌具有选择性作用，而 cv. Tavrida 提取物对革兰氏阴性 和 细菌以及 、 和 真菌具有选择性作用。在 31.3-1000 µg/mL 的浓度范围内，cv. Novichok、cv. Smaragd 和 cv. Citral 提取物会导致线虫死亡。已经确定 cv. Novichok 会影响 UNC-63 蛋白，其分子靶标是 N 型尼古丁受体。
