Kubiliene Asta, Mickute Karolina, Baranauskaite Juste, Marksa Mindaugas, Liekis Arunas, Sadauskiene Ilona
Department of Analytical and Toxicological Chemistry, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu St. 13, LT-50161 Kaunas, Lithuania.
Neuroscience Institute, Lithuanian University of Health Sciences, Eiveniu St. 4, LT-50161 Kaunas, Lithuania.
Life (Basel). 2021 Jul 2;11(7):647. doi: 10.3390/life11070647.
In recent decades, a lot of attention has been paid to L. due to its useful applications, including in fibers, oil, food for humans and animals, and therapeutics. The present study aimed to determine antioxidant activity of cannabinoids in L. in vivo, evaluating the possible antioxidative effect of L. extract (CE) on malondialdehyde (MDA) and glutathione (GSH) concentrations as well as on catalase (CAT) activity in BALB/c mice. In total, 40 mice were divided into five equal groups: the aluminum group (7.5 mg AlCl/kg/d (0.15 LD), the saline group, the 10% ethanol group (an appropriate amount of the solution for mouse weight), the CE group (1.6 mg CE/g/day), and the aluminum-CE group (7.5 mg AlCl plus 1.6 mg CE/g/day). The results of the study showed that CE significantly decreased (by 26.81%, < 0.05) the concentration of GSH in blood of the mice and the concentration of MDA in the brain (by 82.12%) and liver (by 53.5%) of the mice compared to the respective concentrations in the AlCl group. CE significantly ( < 0.05) increased CAT activity in the brain (by 64.79%) and liver (by 72.37%) of the mice after the AlCl-induced prooxidant effect. The results showed the antioxidant activity of cannabidiolic acid (CBDA) in vitro. The findings in vivo indicate that L. is a good source of natural antioxidants and can be used in the management of oxidative stress.
近几十年来，由于其在纤维、油脂、人类和动物食品以及治疗方面的有用应用，人们对[植物名称未明确，用“L.”指代]给予了大量关注。本研究旨在确定[植物名称未明确，用“L.”指代]中大麻素的体内抗氧化活性，评估[植物名称未明确，用“L.”指代]提取物（CE）对BALB/c小鼠丙二醛（MDA）和谷胱甘肽（GSH）浓度以及过氧化氢酶（CAT）活性的可能抗氧化作用。总共40只小鼠被分为五个相等的组：铝组（7.5毫克AlCl/千克/天（0.15 LD））、生理盐水组、10%乙醇组（根据小鼠体重给予适量溶液）、CE组（1.6毫克CE/克/天）和铝-CE组（7.5毫克AlCl加1.6毫克CE/克/天）。研究结果表明，与AlCl组各自的浓度相比，CE显著降低了（降低了26.81%，P<0.05）小鼠血液中GSH的浓度以及小鼠大脑中MDA的浓度（降低了82.12%）和肝脏中MDA的浓度（降低了53.5%）。在AlCl诱导的促氧化作用后，CE显著（P<0.05）提高了小鼠大脑（提高了64.79%）和肝脏（提高了72.37%）中的CAT活性。结果显示了大麻二酚酸（CBDA）的体外抗氧化活性。体内研究结果表明，[植物名称未明确，用“L.”指代]是天然抗氧化剂的良好来源，可用于管理氧化应激。
