Faculty of Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, Iran.
Faculty of Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, Iran.
Int J Pharm. 2020 Nov 15;589:119855. doi: 10.1016/j.ijpharm.2020.119855. Epub 2020 Sep 8.
The aim of this work is to develop dual-functional scaffolds for bone tissue regeneration and local antibiotic delivery applications. In this respect, bioresorbable bredigite (CaMgSiO) porous scaffolds were fabricated by a foam replica method, loaded with vancomycin hydrochloride and encapsulated in poly lactic-co-glycolic acid (PLGA) coatings. Field emission scanning electron microscopy, Archimedes porosimetry and Fourier-transform infrared spectroscopy were used to characterize the structure of the scaffolds. The drug delivery kinetics and cytocompatibility of the prepared scaffolds were also studied in vitro. The bare sample exhibited a burst release of vancomycin and low biocompatibility with respect to dental pulp stem cells based on the MTT assay due to the fast bioresorption of bredigite. While keeping the desirable characteristics of pores for tissue engineering, the biodegradable PLGA coatings modified the drug release kinetics, buffered physiological pH and hence improved the cell viability of the vancomycin-loaded scaffolds considerably.
这项工作的目的是开发用于骨组织再生和局部抗生素递送应用的双重功能支架。在这方面，通过泡沫复制法制备了可生物吸收的钙镁硅（CaMgSiO）多孔支架，负载盐酸万古霉素并封装在聚乳酸-共-羟基乙酸（PLGA）涂层中。用场发射扫描电子显微镜、阿基米德孔隙率法和傅里叶变换红外光谱对支架的结构进行了表征。还研究了制备支架的药物释放动力学和细胞相容性。裸样由于钙镁硅的快速生物吸收，表现出万古霉素的突释和对牙髓干细胞的低生物相容性，根据 MTT 测定。而可生物降解的 PLGA 涂层保持了组织工程中孔的理想特性，改变了药物释放动力学，缓冲了生理 pH 值，从而大大提高了载万古霉素支架的细胞活力。
