Mokkala Kati, Juhila Juuso, Houttu Noora, Sorsa Timo, Laitinen Kirsi
Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turku, Turku, Finland.
Actim Oy, Espoo, Finland.
Heliyon. 2020 Aug 30;6(8):e04788. doi: 10.1016/j.heliyon.2020.e04788. eCollection 2020 Aug.
Lower level of insulin-like growth factor-binding protein (IGFBP-1) has been observed in insulin resistance, while higher level of matrix metalloproteinase-8 (MMP-8) has been linked to obesity. The aim here was to study in overweight and obese women, typically manifesting with insulin resistance, whether IGFBP-1 and MMP-8 are related to and reflect systemic low-grade inflammation, metabolism and diet. Fasting serum from overweight and obese pregnant women (n = 100) in early pregnancy were analysed for IGFBP-1, phosphorylated IGFBP-1 (phIGFBP-1) and MMP-8. High-sensitivity CRP and GlycA were used as markers for low grade inflammation. GlycA and lipids were quantified using NMR. IGFBP-1 associated negatively with GlycA, evidenced by higher concentrations in the lowest quartile (median 1.53 (IQR 1.45-1.72)) compared to the highest (1.46 (1.39-1.55)) (P = 0.03). Several lipid metabolites, particularly HDL-cholesterol, correlated inversely with phIGFBP-1 (FDR<0.1). Nutritional status and diet contributed to the levels of IGFBP-1, demonstrated as an inverse correlation with maternal weight (Spearman r = -0.205, P = 0.04) and dietary intake of vitamin A (r = -0.253, P = 0.014) and a direct correlation with dietary intake of polyunsaturated fatty acids (Spearman r = 0.222, P = 0.03). MMP-8 correlated inversely with pyridoxine (r = -0.321, P = 0.002) and potassium (r = -0.220, P = 0.033). Maternal serum IGFBP-1 may contribute to maternal lipid metabolism in overweight and obese women during early pregnancy. These findings may be of importance in identification of metabolic disturbances preceding the adverse metabolic outcomes in pregnancy.
在胰岛素抵抗中观察到胰岛素样生长因子结合蛋白（IGFBP-1）水平较低，而基质金属蛋白酶-8（MMP-8）水平较高与肥胖有关。本研究的目的是在通常表现为胰岛素抵抗的超重和肥胖女性中，研究IGFBP-1和MMP-8是否与全身低度炎症、代谢和饮食相关并反映这些情况。分析了超重和肥胖孕妇（n = 100）孕早期的空腹血清中的IGFBP-1、磷酸化IGFBP-1（phIGFBP-1）和MMP-8。高敏CRP和GlycA用作低度炎症的标志物。使用核磁共振定量GlycA和脂质。IGFBP-1与GlycA呈负相关，最低四分位数（中位数1.53（IQR 1.45 - 1.72））的浓度高于最高四分位数（1.46（1.39 - 1.55）），证明了这一点（P = 0.03）。几种脂质代谢物，特别是高密度脂蛋白胆固醇，与phIGFBP-1呈负相关（FDR<0.1）。营养状况和饮食对IGFBP-1水平有影响，表现为与孕妇体重呈负相关（斯皮尔曼r = -0.205，P = 0.04）、与维生素A的饮食摄入量呈负相关（r = -0.253，P = 0.014）以及与多不饱和脂肪酸的饮食摄入量呈正相关（斯皮尔曼r = 0.222，P = 0.03）。MMP-8与吡哆醇呈负相关（r = -0.321，P = 0.002）和与钾呈负相关（r = -0.220，P = 0.033）。孕早期超重和肥胖女性的母体血清IGFBP-1可能有助于母体脂质代谢。这些发现可能对识别妊娠不良代谢结局之前的代谢紊乱具有重要意义。
