Department of Prosthodontics, Affiliated Stomatology Hospital, Nanjing Medical University; Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, 210029, People's Republic of China.
Department of Endodontics, Affiliated Stomatology Hospital, Nanjing Medical University; Jiangsu Province Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, 210029, People's Republic of China.
Int J Nanomedicine. 2023 Mar 24;18:1491-1505. doi: 10.2147/IJN.S395631. eCollection 2023.
To investigate the effects of proanthocyanidins (PA), myricetin, resveratrol, and kaempferol on the modification of dentin collagen and the inhibition of matrix metalloproteinase (MMP) activity, and to evaluate their contributions to the biomimetic remineralization and resin-dentin bonding performance.
Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and in situ zymography were applied to verify the collagen modification and MMP activity inhibition induced by these four polyphenols. Scanning electron microscopy/energy dispersive spectrometer (SEM/EDS) analysis, X-ray diffraction (XRD), ATR-FTIR, Vickers hardness numbers (VHN), and micro-computed tomography (micro-CT) were performed to characterize the remineralized dentin. Microtensile bond strength (μTBS) and nanoleakage were investigated to evaluate the effects of the four polyphenols on resin-dentin bonding durability.
ATR-FTIR and in situ zymography confirmed that these four polyphenols could modify dentin collagen and inhibit MMP activity, respectively. Chemoanalytic characterization exhibited the efficacies of the four polyphenols in promoting dentin biomimetic remineralization. The surface hardness of PA-pretreated dentin was the greatest. Micro-CT results demonstrated that the PAs group possessed the highest amount of dentin surface minerals and the lowest amount of deep-layer minerals. The surface and deep-layer mineral contents of the Myr group were higher than Res and Kae groups. Treatment with these four polyphenols significantly increased the initial μTBS compared with the control group without primer conditioning. μTBS decreased significantly during aging, and the decrease was more severe in the PAs and Kae groups than in the Myr and Res groups. With or without aging, the polyphenol groups exhibited relatively less fluorescence. However, the Myr and Res groups showed less serious nanoleakage after aging.
PA, myricetin, resveratrol, and kaempferol can modify dentin collagen, inhibit MMP activity, promote biomimetic remineralization, and improve resin-dentin bond durability. Compared with PA and kaempferol, myricetin and resveratrol are more effective in improving resin-dentin bonding.
研究原花青素（PA）、杨梅素、白藜芦醇和山奈酚对牙本质胶原修饰和基质金属蛋白酶（MMP）活性抑制的影响，并评价它们对仿生再矿化和树脂-牙本质粘结性能的贡献。
采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）和原位酶谱法验证这四种多酚诱导的胶原修饰和 MMP 活性抑制。扫描电子显微镜/能谱仪（SEM/EDS）分析、X 射线衍射（XRD）、ATR-FTIR、维氏硬度数（VHN）和微计算机断层扫描（micro-CT）用于表征再矿化牙本质。微拉伸粘结强度（μTBS）和纳米渗漏用于评价这四种多酚对树脂-牙本质粘结耐久性的影响。
ATR-FTIR 和原位酶谱法证实，这四种多酚可分别修饰牙本质胶原并抑制 MMP 活性。化学分析表明，这四种多酚均能促进牙本质仿生再矿化。PA 预处理牙本质的表面硬度最大。微 CT 结果表明，PA 组具有最高的牙本质表面矿物质含量和最低的深层矿物质含量。Myr 组的表面和深层矿物质含量均高于 Res 和 Kae 组。与未经底漆处理的对照组相比，用这四种多酚处理后，初始 μTBS 显著增加。老化过程中，μTBS 显著降低，PA 和 Kae 组的降低幅度大于 Myr 和 Res 组。无论是否老化，多酚组的荧光均相对较少。然而，Myr 和 Res 组在老化后表现出较轻的纳米渗漏。
PA、杨梅素、白藜芦醇和山奈酚可修饰牙本质胶原、抑制 MMP 活性、促进仿生再矿化，并提高树脂-牙本质粘结耐久性。与 PA 和 kaempferol 相比，myricetin 和 resveratrol 更有效地改善树脂-牙本质粘结。
