Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Molecules. 2020 Sep 12;25(18):4192. doi: 10.3390/molecules25184192.
Overexpression of the histone acetyltransferase and the H NMR spectroscopic experiments of the endophytic fungus resulted in the isolation of two new compounds, monosporasols A () and B (), and two known compounds, pestaloficin C () and arthrinone (). Their planar structures and absolute configurations were determined by spectroscopic analysis including high resolution electrospray ionization mass spectroscopy (HRESIMS), one-dimensional (1D) and two-dimensional (2D) NMR, and calculated electronic circular dichroism data. Compounds were screened in cytotoxic bioassays against HeLa, HCT-8, A549 and MCF-7 cells. Our work highlights the enormous potential of epigenetic manipulation along with the NMR comparison as an effective strategy for unlocking the chemical diversity encoded by fungal genomes.
组蛋白乙酰转移酶的过表达和内生真菌的 1H NMR 光谱实验，导致分离得到两种新化合物，即单孢子醇 A（()）和 B（()），以及两种已知化合物，即 pestaloficin C（()）和 arthrinone（()）。通过包括高分辨电喷雾电离质谱（HRESIMS）、一维（1D）和二维（2D）NMR 以及计算电子圆二色谱数据在内的光谱分析，确定了它们的平面结构和绝对构型。化合物 对 HeLa、HCT-8、A549 和 MCF-7 细胞进行了细胞毒性生物测定筛选。我们的工作强调了表观遗传操作以及 NMR 比较作为一种有效策略的巨大潜力，用于挖掘真菌基因组所编码的化学多样性。
