Sengul Esra, Elitas Meltem
Faculty of Engineering and Natural Sciences, Sabanci University, 34956 Istanbul, Turkey.
Nanotechnology Research and Application Center, Sabanci University, 34956 Istanbul, Turkey.
Micromachines (Basel). 2020 Sep 11;11(9):845. doi: 10.3390/mi11090845.
Integration of microfabricated, single-cell resolution and traditional, population-level biological assays will be the future of modern techniques in biology that will enroll in the evolution of biology into a precision scientific discipline. In this study, we developed a microfabricated cell culture platform to investigate the indirect influence of macrophages on glioma cell behavior. We quantified proliferation, morphology, motility, migration, and deformation properties of glioma cells at single-cell level and compared these results with population-level data. Our results showed that glioma cells obtained slightly slower proliferation, higher motility, and extremely significant deformation capability when cultured with 50% regular growth medium and 50% macrophage-depleted medium. When the expression levels of E-cadherin and Vimentin proteins were measured, it was verified that observed mechanophenotypic alterations in glioma cells were not due to epithelium to mesenchymal transition. Our results were consistent with previously reported enormous heterogeneity of U87 glioma cell line. Herein, for the first time, we quantified the change of deformation indexes of U87 glioma cells using microfluidic devices for single-cells analysis.
将微制造的单细胞分辨率与传统的群体水平生物学检测相结合，将成为现代生物学技术的未来发展方向，推动生物学向精密科学学科发展。在本研究中，我们开发了一个微制造细胞培养平台，以研究巨噬细胞对胶质瘤细胞行为的间接影响。我们在单细胞水平上对胶质瘤细胞的增殖、形态、运动性、迁移和变形特性进行了量化，并将这些结果与群体水平数据进行了比较。我们的结果表明，当胶质瘤细胞在含有50%正常生长培养基和50%巨噬细胞缺失培养基中培养时，其增殖速度略有减慢，运动性增强，变形能力极为显著。在检测E-钙黏蛋白和波形蛋白的表达水平时，证实了胶质瘤细胞中观察到的机械表型改变并非由上皮-间质转化引起。我们的结果与先前报道的U87胶质瘤细胞系的巨大异质性一致。在此，我们首次使用微流控装置对U87胶质瘤细胞的变形指数变化进行了单细胞分析量化。
