Dix B A, Cohen P S, Laux D C, Cleeland R
Department of Chemotherapy, Hoffmann-La Roche Inc., Nutley, New Jersey 07110.
Antimicrob Agents Chemother. 1988 May;32(5):770-2. doi: 10.1128/AAC.32.5.770.
A simple radiochemical method for evaluating the action of antibiotics on Escherichia coli cells in biofilms is reported. After growth, biofilms of E. coli ATCC 25922 on disks of urinary catheter material were suspended in fresh medium containing or lacking an antibiotic, incubated for 4 h at 37 degrees C, and pulse-labeled with [3H]leucine for 5 min. Radioactivity in trichloracetic acid-precipitable material in the biofilm and in the surrounding medium (planktonic E. coli) was then measured. Antibiotic-induced inhibition of incorporation of [3H]leucine into the cells in the biofilm was far less pronounced than incorporation into planktonic cells and, furthermore, correlated well with loss in viable counts. The method is simple, inexpensive, and extremely timesaving.
报道了一种用于评估抗生素对生物膜中大肠杆菌细胞作用的简单放射化学方法。生长后，将导尿管材料圆盘上的大肠杆菌ATCC 25922生物膜悬浮于含有或不含抗生素的新鲜培养基中，于37℃孵育4小时，并用[3H]亮氨酸脉冲标记5分钟。然后测量生物膜和周围培养基（浮游大肠杆菌）中三氯乙酸可沉淀物质的放射性。抗生素诱导的[3H]亮氨酸掺入生物膜中细胞的抑制作用远不如掺入浮游细胞明显，此外，与活菌数的减少密切相关。该方法简单、廉价且极其节省时间。
