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采用符合美国国家临床实验室标准委员会指南的方法检测黏附细菌的抗菌药敏性。

Testing antimicrobial susceptibilities of adherent bacteria by a method that incorporates guidelines of the National Committee for Clinical Laboratory Standards.

作者信息

Domingue G, Ellis B, Dasgupta M, Costerton J W

机构信息

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada.

出版信息

J Clin Microbiol. 1994 Oct;32(10):2564-8. doi: 10.1128/jcm.32.10.2564-2568.1994.

DOI:10.1128/jcm.32.10.2564-2568.1994
PMID:7814499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC264103/
Abstract

Membrane filters (0.22-microns pore size) were colonized with 10(4) CFU of logarithmic-phase bacteria per filter under laminar flow conditions in a Modified Robbins Device. Colonized filters were then placed upon agar dilution plates for MIC determinations. Subsequently, filters were transferred to control plates and incubated to obtain MBCs.

摘要

在改良的Robbins装置中，于层流条件下，用每片含10⁴CFU对数期细菌接种膜过滤器（孔径0.22微米）。然后将接种好的过滤器置于琼脂稀释平板上进行最低抑菌浓度（MIC）测定。随后，将过滤器转移至对照平板并培养以获得最低杀菌浓度（MBC）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b10/264103/c6060eddb40b/jcm00010-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b10/264103/c6060eddb40b/jcm00010-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1b10/264103/c6060eddb40b/jcm00010-0235-a.jpg

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Constitutive high expression of chromosomal beta-lactamase in Pseudomonas aeruginosa caused by a new insertion sequence (IS1669) located in ampD.位于ampD中的一个新插入序列（IS1669）导致铜绿假单胞菌中染色体β-内酰胺酶的组成型高表达。
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