USDA-ARS, National Germplasm Resources Laboratory, Beltsville, Maryland, United States of America.
USDA-APHIS-PPQ, Plant Germplasm Quarantine Program, Beltsville, Maryland, United States of America.
PLoS One. 2020 Sep 17;15(9):e0239199. doi: 10.1371/journal.pone.0239199. eCollection 2020.
Miscanthus sinensis is a grass used for sugarcane breeding and bioenergy production. Using high throughput sequencing technologies, we identified a new viral genome in infected M. sinensis leaf tissue displaying yellow fleck symptoms. This virus is most related to members of the genus Polerovirus in the family Luteoviridae. The canonical ORFs were computationally identified, the P3 coat protein was expressed, and virus-like particles were purified and found to conform to icosahedral shapes, characteristic of the family Luteoviridae. We propose the name Miscanthus yellow fleck virus for this new virus.
中国芒是一种用于甘蔗育种和生物能源生产的草。利用高通量测序技术，我们在表现出黄斑症状的受感染中国芒叶片组织中鉴定出一种新的病毒基因组。该病毒与杆状病毒科黄花叶病毒属的成员最为相关。通过计算预测了其典型的 ORF 区，表达了 P3 外壳蛋白，并纯化了病毒样颗粒，发现其符合杆状病毒科的二十面体形状特征。我们提议将这种新病毒命名为中国芒黄斑病毒。
