Sodré Camila Stofella, Rodrigues Paulo Matheus Guerra, Vieira Mayra Stambovsky, Marques Paes da Silva Alexandre, Gonçalves Lucio Souza, Ribeiro Marcia Gonçalves, de Carvalho Ferreira Dennis
Faculty of Medicine, Department of Clinical Medicine, Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.
Laboratory of Oral and Systemic Infections, Faculty of Dentistry, Estácio de Sá University- UNESA, Rio de Janeiro, Brazil.
J Oral Microbiol. 2020 Aug 17;12(1):1807179. doi: 10.1080/20002297.2020.1807179.
Oral mycobiome profiling is important to understand host-pathogen interactions that occur in various diseases. Invasive fungal infections are particularly relevant for patients who have received chemotherapy and for those who have HIV infection. In addition, changes in fungal microbiota are associated with the worsening of chronic conditions like atopic dermatitis (AD). This work aims, through a systematic review, to analyze the methods used in previous studies to identify oral fungi and their most frequent species in patients with the following conditions: HIV infection, leukemia, and atopic dermatitis.
A literature search was performed on several different databases. Inclusion criteria were: written in English or Portuguese; published between September 2009 and September 2019; analyzed oral fungi of HIV-infected, leukemia, or AD patients.
21 studies were included and the most identified species was . The predominant methods of identification were morphological (13/21) and sugar fermentation and assimilation tests (11/21). Polymerase chain reaction (PCR) was the most used molecular method (8/21) followed by sequencing techniques (3/21).
Although morphological and biochemical tests are still used, they are associated with high-throughput sequencing techniques, due to their accuracy and time saving for profiling the predominant species in oral mycobiome.
口腔微生物群分析对于理解各种疾病中发生的宿主-病原体相互作用很重要。侵袭性真菌感染对于接受化疗的患者和艾滋病毒感染者尤为重要。此外，真菌微生物群的变化与特应性皮炎（AD）等慢性疾病的恶化有关。这项工作旨在通过系统综述，分析先前研究中用于识别以下疾病患者口腔真菌及其最常见种类的方法：艾滋病毒感染、白血病和特应性皮炎。
在几个不同的数据库中进行了文献检索。纳入标准为：用英文或葡萄牙文撰写；2009年9月至2019年9月期间发表；分析了艾滋病毒感染、白血病或特应性皮炎患者的口腔真菌。
纳入了21项研究，最常鉴定出的种类是 。主要的鉴定方法是形态学方法（13/21）以及糖发酵和同化试验（11/21）。聚合酶链反应（PCR）是最常用的分子方法（8/21），其次是测序技术（3/21）。
尽管形态学和生化试验仍在使用，但由于它们在分析口腔微生物群中主要种类时具有准确性和省时性，因此与高通量测序技术相关。
