Department of Prosthodontics, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.
Stomatological Hospital of Xiamen Medical College, Xiamen, China.
Int J Oral Sci. 2020 Sep 21;12(1):25. doi: 10.1038/s41368-020-00094-1.
Bone tissue engineering has emerged as a promising alternative therapy for patients who suffer bone fractures or defects caused by trauma, congenital diseases or tumours. However, the reconstruction of bone defects combined with osteoporosis remains a great challenge for clinicians and researchers. Based on our previous study, Ca-Si-based bioceramics (MSCs) showed enhanced bone formation capabilities under normal conditions, and strontium was demonstrated to be therapeutic in promoting bone quality in osteoporosis patients. Therefore, in the present study, we attempted to enlarge the application range of MSCs with Sr incorporation in an osteoporotic bone regeneration model to evaluate whether Sr could assist in regeneration outcomes. In vitro readout suggested that Sr-incorporated MSC scaffolds could enhance the expression level of osteogenic and angiogenic markers of osteoporotic bone mesenchymal stem cells (OVX BMSCs). Animal experiments showed a larger new bone area; in particular, there was a tendency for blood vessel formation to be enhanced in the Sr-MSC scaffold group, showing its positive osteogenic capacity in bone regeneration. This study systematically illustrated the effective delivery of a low-cost therapeutic Sr agent in an osteoporotic model and provided new insight into the treatment of bone defects in osteoporosis patients.
骨组织工程已成为一种有前途的替代疗法，适用于因创伤、先天疾病或肿瘤而导致骨折或骨缺损的患者。然而，对于临床医生和研究人员来说，结合骨质疏松症的骨缺损重建仍然是一个巨大的挑战。基于我们之前的研究，钙硅基生物陶瓷（MSCs）在正常条件下表现出增强的成骨能力，锶已被证明在治疗骨质疏松症患者的骨质量方面具有疗效。因此，在本研究中，我们试图在骨质疏松症骨再生模型中通过掺入 Sr 来扩大 MSCs 的应用范围，以评估 Sr 是否有助于再生结果。体外检测结果表明，Sr 掺入 MSC 支架可以增强骨质疏松症骨髓间充质干细胞（OVX BMSCs）成骨和成血管标志物的表达水平。动物实验显示出更大的新骨区域；特别是，Sr-MSC 支架组的血管形成有增强的趋势，显示出其在骨再生中的阳性成骨能力。本研究系统地说明了在骨质疏松模型中有效传递低成本治疗性 Sr 剂，并为骨质疏松症患者的骨缺损治疗提供了新的见解。
