Schäpe Stephanie Serena, Krause Jannike Lea, Masanetz Rebecca Katharina, Riesbeck Sarah, Starke Robert, Rolle-Kampczyk Ulrike, Eberlein Christian, Heipieper Hermann-Josef, Herberth Gunda, von Bergen Martin, Jehmlich Nico
Department of Molecular Systems Biology, Helmholtz-Centre for Environmental Research GmbH - UFZ, 04318 Leipzig, Germany.
Department of Environmental Immunology, Helmholtz-Centre for Environmental Research GmbH - UFZ, 04318 Leipzig, Germany.
Microorganisms. 2020 Sep 19;8(9):1436. doi: 10.3390/microorganisms8091436.
Bisphenol S (BPS) is an industrial chemical used in the process of polymerization of polycarbonate plastics and epoxy resins and thus can be found in various plastic products and thermal papers. The microbiota disrupting effect of BPS on the community structure of the microbiome has already been reported, but little is known on how BPS affects bacterial activity and function. To analyze these effects, we cultivated the simplified human intestinal microbiota (SIHUMIx) in bioreactors at a concentration of 45 µM BPS. By determining biomass, growth of SIHUMIx was followed but no differences during BPS exposure were observed. To validate if the membrane composition was affected, fatty acid methyl esters (FAMEs) profiles were compared. Changes in the individual membrane fatty acid composition could not been described; however, the saturation level of the membranes slightly increased during BPS exposure. By applying targeted metabolomics to quantify short-chain fatty acids (SCFA), it was shown that the activity of SIHUMIx was unaffected. Metaproteomics revealed temporal effect on the community structure and function, showing that BPS has minor effects on the structure or functionality of SIHUMIx.
双酚S（BPS）是一种工业化学品，用于聚碳酸酯塑料和环氧树脂的聚合过程，因此可在各种塑料制品和热敏纸中找到。BPS对微生物群落结构的微生物群破坏作用已有报道，但关于BPS如何影响细菌活性和功能知之甚少。为了分析这些影响，我们在生物反应器中以45µM BPS的浓度培养简化的人类肠道微生物群（SIHUMIx）。通过测定生物量来跟踪SIHUMIx的生长，但在BPS暴露期间未观察到差异。为了验证膜组成是否受到影响，比较了脂肪酸甲酯（FAMEs）谱。无法描述单个膜脂肪酸组成的变化；然而，在BPS暴露期间，膜的饱和度略有增加。通过应用靶向代谢组学来定量短链脂肪酸（SCFA），结果表明SIHUMIx的活性未受影响。元蛋白质组学揭示了对群落结构和功能的时间效应，表明BPS对SIHUMIx的结构或功能影响较小。
