sp. nov., a novel bacterium in the family , isolated from marine sediment.

A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238, was isolated from marine sediment sampled on Meishan Island located in the East China Sea. Strain NBU1238 was able to grow optimally at 28-32 °C, at pH 7.5 and with no NaCl. Catalase and oxidase activities, HS production and hydrolysis of Tweens 40 and 60 were positive. Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative. The major cellular fatty acids were iso-C, C, C 9 and C. The only respiratory quinone was menaquinone-9. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine. The 16S rRNA gene sequence of strain NBU1238 showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to GKX, G-1-1-1, MC 3726 and A4T-83, respectively. Phylogenetic analyses indicated that strain NBU1238 clustered with the genus and was closely related to strains GKX, MC 3726 and G-1-1-1. The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238 and related species of genus were well below the threshold limit for prokaryotic species delineation. The DNA G+C content of the genomic DNA was 65.0 mol%. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238 is considered to be a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NBU1238 (=KCTC 82227=MCCC 1K04772).