Mizoguchi A, Ishizaki H, Nagasawa H, Kataoka H, Isogai A, Tamura S, Suzuki A, Fujino M, Kitada C
Mol Cell Endocrinol. 1987 Jun;51(3):227-35. doi: 10.1016/0303-7207(87)90032-3.
Monoclonal antibodies were produced by immunizing mice with a synthetic decapeptide corresponding to the N-terminal portion of the A-chain of bombyxin, a peptide from Bombyx mori which activates the prothoracic glands of the saturniid moth, Samia cynthia ricini, and was previously called 4K-PTTH. We obtained a hybridoma clone secreting an antibody that recognized specifically bombyxin after treatments for disulfide-bond reduction but did not when untreated. Immunoblotting studies demonstrated the presence of highly heterogeneous immunoreactive components in Bombyx brain homogenates. Immunohistochemistry using this antibody indicated that bombyxin was produced by four pairs of mid-dorsal neurosecretory cells of the brain and transferred to and released from the corpora allata of Bombyx.
用一种合成十肽免疫小鼠制备单克隆抗体，该十肽对应家蚕素A链的N端部分，家蚕素是来自家蚕的一种肽，可激活蓖麻蚕的前胸腺，以前称为4K-PTTH。我们获得了一个杂交瘤克隆，其分泌的抗体在经过二硫键还原处理后能特异性识别家蚕素，而未经处理时则不能。免疫印迹研究表明，家蚕脑匀浆中存在高度异质的免疫反应性成分。使用该抗体的免疫组织化学表明，家蚕素由脑的四对中背神经分泌细胞产生，并转移至家蚕的咽侧体并从其释放。
