Centro de investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, , Morelos, México.
Laboratorio de Helmintología, Centro Nacional de Investigación Disciplinaria en Salud Animal E Inocuidad, INIFAP, Carretera Federal Cuernavaca-Cuautla No. 8534 Col. Progreso, C. P. 62550, Jiutepec, Morelos, México.
Arch Microbiol. 2021 Mar;203(2):549-559. doi: 10.1007/s00203-020-02051-2. Epub 2020 Sep 27.
In the present study, the nematicidal activity of an isolated strain of Mimosa pudica nodules was evaluated against the Nacobbus aberrans (J2) phytonymatodes with a mortality of 88.8%, while against the gastrointestinal nematode Haemonchus contortus (L3) and free-living Panagrellus redivivus was 100%. The ability to inhibit the growth of phytopathogenic fungi Fusarium sp., and Alternaria solani, as well as the oomycete Phytophthora capsici, this antifungal activity may be related to the ability to produce cellulases, siderophores and chitinases by this bacterial strain. Another important finding was the detection of plant growth promoter characteristics, such as auxin production and phosphate solubilization. The strain identified by sequences of the 16S and rpoB genes as Serratia sp. is genetically related to Serratia marcescens and Serratia nematodiphila. The promoter activity of plant growth, antifungal and nematicide of the Serratia sp. strain makes it an alternative for the biocontrol of fungi and nematodes that affect both the livestock and agricultural sectors, likewise, candidate as a growth-promoting bacterium.
在本研究中，评估了含羞草根瘤菌分离株对 Nacobbus aberrans（J2）线虫的杀线虫活性，死亡率为 88.8%，而对胃肠道线虫捻转血矛线虫（L3）和自由生活的潘格尔勒斯雷迪维乌斯（Panagrellus redivivus）的杀线虫活性为 100%。该菌株还具有抑制植物病原菌镰刀菌（Fusarium sp.）和茄腐镰刀菌（Alternaria solani）以及卵菌疫霉属（Phytophthora capsici）生长的能力，这种抗真菌活性可能与该菌株产生纤维素酶、铁载体和几丁质酶的能力有关。另一个重要发现是检测到植物生长促进剂的特性，如生长素的产生和磷酸盐的溶解。通过 16S 和 rpoB 基因序列鉴定的菌株为沙雷氏菌属（Serratia sp.），与粘质沙雷氏菌（Serratia marcescens）和杀线虫沙雷氏菌（Serratia nematodiphila）在遗传上有关。沙雷氏菌属（Serratia sp.）菌株的植物生长促进、抗真菌和杀线虫活性使其成为防治影响畜牧业和农业部门的真菌和线虫的生物防治剂的替代品，同时也可作为一种促进生长的细菌。
