Vizzini Priya, Beltrame Elena, Zanet Valentina, Vidic Jasmina, Manzano Marisa
Department of Agriculture Food Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy.
Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Foods. 2020 Sep 24;9(10):1353. doi: 10.3390/foods9101353.
To answer to food industry requests to monitor the presence of in cold-smoked salmon samples and to extend their shelf-life, a qPCR protocol for the detection of , and an antibacterial active packaging reinforced with zinc magnesium oxide nanoparticles (Zn-MgO NPs) were developed. The qPCR allowed the sensitive and easy detection of in naturally contaminated samples, with specificity in full agreement with the standard methods. The halo diffusion study indicated a high antibacterial efficiency of 1 mg/mL Zn-MgO NPs against , while the flow cytometry showed only moderate cytotoxicity of the nanoparticles towards mammalian cells at a concentration above 1 mg/mL. Thus, the novel active packaging was developed by using 1 mg/mL of Zn-MgO NPs to reinforce the alginate film. Cold-smoked salmon samples inoculated with and air-packed with the Zn-MgO NPs-alginate nanobiocomposite film showed no bacterial proliferation at 4 °C during 4 days. In the same condition, growth in control contaminated samples packed with alginate film alone. Our results suggest that Zn-MgO nanoparticles can extend the shelf-life of cold-smoked salmon samples.
为响应食品行业监测冷熏三文鱼样品中[具体细菌名称未给出]的存在并延长其保质期的要求，开发了一种用于检测[具体细菌名称未给出]的qPCR方法，以及一种用锌镁氧化物纳米颗粒（Zn-MgO NPs）增强的抗菌活性包装。该qPCR方法能够灵敏且简便地检测天然污染样品中的[具体细菌名称未给出]，其特异性与标准方法完全一致。光晕扩散研究表明，1 mg/mL的Zn-MgO NPs对[具体细菌名称未给出]具有较高的抗菌效率，而流式细胞术显示，在浓度高于1 mg/mL时，纳米颗粒对哺乳动物细胞仅具有中等细胞毒性。因此，通过使用1 mg/mL的Zn-MgO NPs增强海藻酸盐薄膜，开发了新型活性包装。接种[具体细菌名称未给出]并用Zn-MgO NPs-海藻酸盐纳米生物复合薄膜气调包装的冷熏三文鱼样品在4℃下4天内未出现细菌增殖。在相同条件下，仅用海藻酸盐薄膜包装的对照污染样品中有[具体细菌名称未给出]生长。我们的结果表明，Zn-MgO纳米颗粒可以延长冷熏三文鱼样品的保质期。
