大肠杆菌被设计用来以 CO 和甲酸为碳源生长。
Escherichia coli is engineered to grow on CO and formic acid.
机构信息
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, KAIST, Daejeon, Republic of Korea.
出版信息
Nat Microbiol. 2020 Dec;5(12):1459-1463. doi: 10.1038/s41564-020-00793-9. Epub 2020 Sep 28.
We engineered Escherichia coli to grow on CO and formic acid alone by introducing the synthetic CO and formic acid assimilation pathway, expressing two formate dehydrogenase genes, fine-tuning metabolic fluxes and optimizing the levels of cytochrome bo and bd-I ubiquinol oxidase. Our engineered strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO and formic acid alone.
我们通过引入合成的 CO 和甲酸同化途径、表达两种甲酸脱氢酶基因、微调代谢通量以及优化细胞色素 bo 和 bd-I 泛醌氧化酶的水平，成功地使大肠杆菌能够仅利用 CO 和甲酸进行生长。我们的工程菌株在 450 小时内可达到 600nm 处的光密度 7.38，并且有望成为一种仅利用 CO 和甲酸进行生长的平台菌株。
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