孕妇沙眼衣原体感染非培养检测方法的比较

A comparison of nonculture-dependent methods for detection of Chlamydia trachomatis infections in pregnant women.

作者信息

Baselski V S, McNeeley S G, Ryan G, Robison M

出版信息

Obstet Gynecol. 1987 Jul;70(1):47-52.

Abstract

Two nonculture-dependent methods for the detection of Chlamydia trachomatis in endocervical samples from obstetric patients were compared with routine isolation in McCoy cell cultures. When compared with culture, the sensitivities and specificities of the methods were: direct fluorescent antibody staining (MicroTrak [Syva Co.]) 98.1 and 95.4%, and enzyme immunoassay (Chlamydiazyme [Abbott Laboratories]) 96.3 and 92.9%, respectively. In 89% of apparent false-positive direct fluorescent antibody cases and 64% of enzyme immunoassay cases, an additional positive nonculture result was considered to indicate infection missed by culture. Considering these data, revised sensitivities were 84.4% for culture, 95.2% for direct fluorescent antibody, and 95.3% for enzyme immunoassay. Revised specificities were 99.5% for direct fluorescent antibody and 97.3% for enzyme immunoassay. Both nonculture tests appear acceptable for screening high-risk obstetric patients, and may be more sensitive than routine cell culture.

摘要

将两种用于检测产科患者宫颈样本中沙眼衣原体的非培养方法与在 McCoy 细胞培养中的常规分离方法进行了比较。与培养法相比，这两种方法的敏感性和特异性分别为：直接荧光抗体染色法（MicroTrak [Syva 公司]）98.1% 和 95.4%，酶免疫测定法（Chlamydiazyme [雅培实验室]）96.3% 和 92.9%。在 89% 的明显直接荧光抗体假阳性病例和 64% 的酶免疫测定假阳性病例中，另一个非培养阳性结果被认为表明培养法漏检了感染。考虑到这些数据，培养法的修正敏感性为 84.4%，直接荧光抗体法为 95.2%，酶免疫测定法为 95.3%。直接荧光抗体法的修正特异性为 99.5%，酶免疫测定法为 97.3%。两种非培养检测方法对于筛查高危产科患者似乎都是可接受的，并且可能比常规细胞培养更敏感。