Department of Biochemistry, Maharshi Dayanad University, Rohtak, Haryana, 124001, India.
Centre for Medical Biotechnology, Maharshi Dayanad University, Rohtak, Haryana, 124001, India.
Bioprocess Biosyst Eng. 2018 Mar;41(3):313-329. doi: 10.1007/s00449-017-1878-8. Epub 2017 Dec 19.
Determination of hydrogen peroxide (HO) has become essential in pharmaceutical, biological, clinical and environmental studies. The conventional detection methods of HO such as colourimetry, titration, chromatography, spectrophotometry, fluorimetry, chemiluminescence have limited success, due to their poor selectivity and sensitivity, long analysis time and lack of long-term reliability and reproducibility. The biosensors overcome these limitations because of their simplicity, rapidity, selectivity and high sensitivity. This review describes the principle, analytic parameters, merits and demerits of various methods of HO determination with special emphasis on biosensors. The classification of biosensors based on various materials/nanomaterials and electrodes have been described in detail. The recent advances in vivo sensing and bio-sensing of HO by hemoglobin nanoparticles are also presented. The significant challenges and future perspective for highly selective HO detection are discussed.
过氧化氢（HO）的测定在药物、生物、临床和环境研究中变得至关重要。由于其选择性和灵敏度差、分析时间长以及缺乏长期可靠性和可重复性，HO 的传统检测方法，如比色法、滴定法、色谱法、分光光度法、荧光法、化学发光法，都有一定的局限性。由于其简单、快速、选择性和高灵敏度，生物传感器克服了这些限制。本文综述了 HO 测定的各种方法的原理、分析参数、优缺点，特别强调了生物传感器。详细描述了基于各种材料/纳米材料和电极的生物传感器的分类。还介绍了血红蛋白纳米粒子在体内 HO 传感和生物传感方面的最新进展。讨论了高选择性 HO 检测的重大挑战和未来展望。
