Martinez Diego Stéfani T, Da Silva Gabriela H, de Medeiros Aline Maria Z, Khan Latif U, Papadiamantis Anastasios G, Lynch Iseult
Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas 13083-100, Sao Paulo, Brazil.
School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Nanomaterials (Basel). 2020 Sep 29;10(10):1936. doi: 10.3390/nano10101936.
In this work, we evaluated the effect of protein corona formation on graphene oxide (GO) mixture toxicity testing (i.e., co-exposure) using the model and assessing acute toxicity determined as immobilisation. Cadmium (Cd) and bovine serum albumin (BSA) were selected as co-pollutant and protein model system, respectively. Albumin corona formation on GO dramatically increased its colloidal stability (ca. 60%) and Cd adsorption capacity (ca. 4.5 times) in reconstituted water ( medium). The acute toxicity values (48 h-EC) observed were 0.18 mg L for Cd-only and 0.29 and 0.61 mg L following co-exposure of Cd with GO and BSA@GO materials, respectively, at a fixed non-toxic concentration of 1.0 mg L. After coronation of GO with BSA, a reduction in cadmium toxicity of 110 % and 238% was achieved when compared to bare GO and Cd-only, respectively. Integration of datasets associated with graphene-based materials, heavy metals and mixture toxicity is essential to enable re-use of the data and facilitate nanoinformatics approaches for design of safer nanomaterials for water quality monitoring and remediation technologies. Hence, all data from this work were annotated and integrated into the NanoCommons Knowledge Base, connecting the experimental data to nanoinformatics platforms under the FAIR data principles and making them interoperable with similar datasets.
在这项工作中，我们使用该模型评估了蛋白质冠形成对氧化石墨烯（GO）混合物毒性测试（即共同暴露）的影响，并通过固定化评估急性毒性。分别选择镉（Cd）和牛血清白蛋白（BSA）作为共同污染物和蛋白质模型系统。在再生水（介质）中，GO上白蛋白冠的形成显著提高了其胶体稳定性（约60%）和Cd吸附能力（约4.5倍）。在固定无毒浓度为1.0 mg/L的情况下，观察到的急性毒性值（48 h-EC）对于仅Cd为0.18 mg/L，Cd与GO和BSA@GO材料共同暴露后分别为0.29和0.61 mg/L。GO与BSA形成冠后，与裸GO和仅Cd相比，镉毒性分别降低了110%和238%。整合与基于石墨烯的材料、重金属和混合物毒性相关的数据集对于数据的再利用以及促进用于水质监测和修复技术的更安全纳米材料设计的纳米信息学方法至关重要。因此，这项工作的所有数据都经过注释并整合到纳米公共知识库中，根据FAIR数据原则将实验数据连接到纳米信息学平台，并使其与类似数据集可互操作。
