迁移抑制因子与血液凝固系统：去纤维蛋白、肝素和凝血酶的作用
Migration inhibition factor and the blood clotting system: effects of defibrination, heparin and thrombin.
作者信息
Mäkinen T, Tötterman T H, Gordin A, Weber T H
出版信息
Clin Exp Immunol. 1977 Jul;29(1):181-6.
Heparin is not suitable as an anticoagulant in the leucocyte migration test used to demonostrate the presence of migration inhibition factor (MIF) due to a rapid disappearance of the response after even a short storage of the blood. The use of defibrinated blood is highly recommended and defibrinated blood can be stored for at least 90 min without any diminution of the response. The change in response when using heparinized blood is not due to any direct effect of heparin, because heparin has no effect when added to defibrinated blood. However, heparin, added together with thrombin, is capable of abolishing the MIF effect completely. The basis for this phenomenon is most probably the binding of the heparin-antithrombin cofactor (AT III) to a complex with heparin and thrombin. The activity of MIF requires the presence of AT III, its esterase-inhibiting activity probably being crucial, in order to express MIF activity on macrophages. This mechanism forms a link between certain cellular immune reactions and the blood-clotting system.
在用于证明迁移抑制因子（MIF）存在的白细胞迁移试验中，肝素不适宜作为抗凝剂，因为即使血液储存时间很短，反应也会迅速消失。强烈建议使用去纤维蛋白血，去纤维蛋白血可储存至少90分钟而反应无任何减弱。使用肝素化血液时反应的变化并非由于肝素的任何直接作用，因为将肝素添加到去纤维蛋白血中时并无作用。然而，肝素与凝血酶一起添加时，能够完全消除MIF效应。这种现象的基础很可能是肝素 - 抗凝血酶辅因子（AT III）与肝素和凝血酶的复合物结合。MIF的活性需要AT III的存在，其酯酶抑制活性可能至关重要，以便在巨噬细胞上表达MIF活性。这种机制在某些细胞免疫反应和血液凝固系统之间形成了联系。
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