Moussa Hoda, Jones Megan M, Huo Ningbo, Zhang Runsheng, Keskar Mayuresh, Visser Michelle B, Swihart Mark T, Cheng Chong, Sabatini Camila
Department of Chemistry, Buffalo State College, 1300 Elmwood Avenue, Buffalo, NY, 14222, USA.
Department of Oral Biology, School of Dental Medicine, University at Buffalo, 3435 Main Street, Buffalo, NY, 14214, USA.
Clin Oral Investig. 2021 May;25(5):2877-2889. doi: 10.1007/s00784-020-03605-w. Epub 2020 Oct 2.
This study investigated the antibacterial, cytotoxicity, and mechanical properties of a dental adhesive modified with quaternary ammonium monomer ((2-acryloyloxyethyl)dimethyldodecylammonium bromide) and cross-linker (bis(2-acryloyloxyethyl)methyldodecylammonium bromide).
Monomer (M), cross-linker (C), or a combination of these (M + C) were incorporated into adhesive Adper Single Bond Plus (SB) in 5, 10, or 25% (as wt%). A colony-forming unit and MTT assays were used to evaluate antibacterial properties against Streptococcus mutans and cell viability. Resin-dentin beams (0.9 ± 0.1 mm) were evaluated for micro-tensile bond strength (μTBS) after 24 h, 6 months, and 3 years. Hourglass specimens were evaluated for ultimate tensile strength (UTS) after 24 h, 1 week, and 6 months. Micro-hardness measurements after softening in ethanol were taken as an indirect assessment of the polymer cross-linking density. Kruskal-Wallis, one-way ANOVA, two-way ANOVA, and Student's t test were used for analysis of the antibacterial, cytotoxicity, μTBS, UTS, and hardness data, all with a significance level of p < 0.05.
10%M and 25%M demonstrated a significant reduction in S. mutans relative to SB (p < 0.001). No differences in cytotoxicity were detected for any of the groups. After 6 months, no changes in μTBS were shown for any of the groups. After 3 years, all groups evidenced a significant decrease in μTBS (p < 0.05) except 5%M, 5%C, and 5%M + 5%C. All groups demonstrated either stable or significantly increased UTS after 6 months. Except for the cross-linker groups, a significant decrease in micro-hardness was shown for all groups after softening in ethanol (p < 0.05).
A 5-10% of monomer may render the resin antibacterial without a compromise to its mechanical and bonding properties.
Biomodification of a resin adhesive with an antibacterial monomer and cross-linker may help improve the life span of adhesive restorations.
本研究调查了用季铵单体（（2-丙烯酰氧基乙基）二甲基十二烷基溴化铵）和交联剂（双（2-丙烯酰氧基乙基）甲基十二烷基溴化铵）改性的牙科粘合剂的抗菌、细胞毒性和机械性能。
将单体（M）、交联剂（C）或它们的组合（M + C）以5%、10%或25%（重量）的比例加入到Adper Single Bond Plus（SB）粘合剂中。采用菌落形成单位和MTT试验评估对变形链球菌的抗菌性能和细胞活力。对树脂-牙本质梁（0.9±0.1毫米）在24小时、6个月和3年后进行微拉伸粘结强度（μTBS）评估。对沙漏形试样在24小时、1周和6个月后进行极限拉伸强度（UTS）评估。在乙醇中软化后进行显微硬度测量，作为聚合物交联密度的间接评估。采用Kruskal-Wallis检验、单因素方差分析、双因素方差分析和学生t检验分析抗菌、细胞毒性、μTBS、UTS和硬度数据，所有分析的显著性水平均为p < 0.05。
相对于SB，10%M和25%M组的变形链球菌数量显著减少（p < 0.001）。所有组的细胞毒性均未检测到差异。6个月后，所有组的μTBS均未显示变化。3年后，除5%M、5%C和5%M + 5%C组外，所有组的μTBS均显著降低（p < 0.05）。6个月后，所有组的UTS均显示稳定或显著增加。除交联剂组外，所有组在乙醇中软化后的显微硬度均显著降低（p < 0.05）。
5-10%的单体可使树脂具有抗菌性能，同时不损害其机械性能和粘结性能。
用抗菌单体和交联剂对树脂粘合剂进行生物改性可能有助于提高粘结修复体的使用寿命。
