Evaluation of the antibacterial activity of dental adhesive containing biogenic silver nanoparticles decorated nanographene oxide nanocomposites (Ag@nGO NCs) and effect on bond strength to dentine.

Our study aimed to evaluate the antibacterial activities and dentin bond strengths of silver nanoparticles (Ag NPs) and silver nano-graphene oxide nanocomposites (Ag@nGO NCs) produced by green and chemical synthesis methods added to the dental adhesive. Ag NPs were produced by green synthesis (biogenic) (B-Ag NPs) and chemical synthesis methods (C-Ag NPs) and deposited on nGO (nano-graphene oxide). Ag NPs and Ag@nGO NCs (0.05% w/w) were added to the primer and bond (Clearfil SE Bond). Group 1: control, Group 2: nGO, Group 3: B-Ag NPs, Group 4: B-Ag@nGO NCs, Group 5: C-Ag NPs, Group 6: C-Ag@nGO NCs. Streptococcus mutans (S. mutans) live/dead assay analysis, MTT metabolic activity test, agar disc diffusion test, lactic acid production, and colony forming units (CFUs) tests were performed. Bond strength values were determined by the microtensile bond strength test (μTBS). Failure types were determined by evaluating with SEM. Statistical analysis was performed using one-way ANOVA and two-way ANOVA (p < 0.05). There was a difference between the groups in the viable bacteria ratio and lactic acid production tests (p < 0.05). When the inhibition zone and S. mutans CFUs were evaluated, there was no difference between Group 3 and Group 4 (p > 0.05), but there was a difference between the other groups (p < 0.05). When the metabolic activity of S. mutans was evaluated, there was a difference between Group 1 and other groups, and between Group 2 and Group 5, and Group 6 (p < 0.05). There was no difference between the groups in the μTBS values (p > 0.05). As a result, although the antibacterial activity of B-Ag NPs and B-Ag@nGO Ag NPs obtained by green synthesis is lower than that of chemically synthesis obtained C-Ag NPs and C-Ag@nGO NCs, they provided higher antibacterial activity compared to the control group and did not reduce μTBS. The addition of biogenic Ag NPs to the adhesive system increased the antibacterial effect by maintaining the bond strength of the adhesive. Antibacterial adhesives can increase the restoration life by protecting the tooth-adhesive interface.