Department of Restorative Dentistry, Faculty of Dentistry, Erciyes University, Kayseri, Turkey.
Department of Restorative Dentistry, Faculty of Dentistry, Nuh Naci Yazgan University, Kayseri, Turkey.
Odontology. 2024 Apr;112(2):341-354. doi: 10.1007/s10266-023-00836-7. Epub 2023 Jul 12.
Our study aimed to evaluate the antibacterial activities and dentin bond strengths of silver nanoparticles (Ag NPs) and silver nano-graphene oxide nanocomposites (Ag@nGO NCs) produced by green and chemical synthesis methods added to the dental adhesive. Ag NPs were produced by green synthesis (biogenic) (B-Ag NPs) and chemical synthesis methods (C-Ag NPs) and deposited on nGO (nano-graphene oxide). Ag NPs and Ag@nGO NCs (0.05% w/w) were added to the primer and bond (Clearfil SE Bond). Group 1: control, Group 2: nGO, Group 3: B-Ag NPs, Group 4: B-Ag@nGO NCs, Group 5: C-Ag NPs, Group 6: C-Ag@nGO NCs. Streptococcus mutans (S. mutans) live/dead assay analysis, MTT metabolic activity test, agar disc diffusion test, lactic acid production, and colony forming units (CFUs) tests were performed. Bond strength values were determined by the microtensile bond strength test (μTBS). Failure types were determined by evaluating with SEM. Statistical analysis was performed using one-way ANOVA and two-way ANOVA (p < 0.05). There was a difference between the groups in the viable bacteria ratio and lactic acid production tests (p < 0.05). When the inhibition zone and S. mutans CFUs were evaluated, there was no difference between Group 3 and Group 4 (p > 0.05), but there was a difference between the other groups (p < 0.05). When the metabolic activity of S. mutans was evaluated, there was a difference between Group 1 and other groups, and between Group 2 and Group 5, and Group 6 (p < 0.05). There was no difference between the groups in the μTBS values (p > 0.05). As a result, although the antibacterial activity of B-Ag NPs and B-Ag@nGO Ag NPs obtained by green synthesis is lower than that of chemically synthesis obtained C-Ag NPs and C-Ag@nGO NCs, they provided higher antibacterial activity compared to the control group and did not reduce μTBS. The addition of biogenic Ag NPs to the adhesive system increased the antibacterial effect by maintaining the bond strength of the adhesive. Antibacterial adhesives can increase the restoration life by protecting the tooth-adhesive interface.
我们的研究旨在评估通过绿色和化学合成方法制备的银纳米粒子（Ag NPs）和银纳米石墨烯氧化物纳米复合材料（Ag@nGO NCs）添加到牙科胶粘剂中的抗菌活性和牙本质结合强度。Ag NPs 通过绿色合成（生物合成）（B-Ag NPs）和化学合成方法（C-Ag NPs）制备，并沉积在 nGO（纳米石墨烯氧化物）上。Ag NPs 和 Ag@nGO NCs（0.05％w/w）被添加到底漆和粘结剂（Clearfil SE Bond）中。第 1 组：对照，第 2 组：nGO，第 3 组：B-Ag NPs，第 4 组：B-Ag@nGO NCs，第 5 组：C-Ag NPs，第 6 组：C-Ag@nGO NCs。进行了变形链球菌（S. mutans）死活分析、MTT 代谢活性试验、琼脂圆盘扩散试验、乳酸生成和菌落形成单位（CFU）试验。通过微拉伸结合强度试验（μTBS）确定结合强度值。通过 SEM 评估确定失效类型。采用单因素方差分析和双因素方差分析（p<0.05）进行统计分析。在活菌率和乳酸生成试验中，各组之间存在差异（p<0.05）。当评价抑菌带和 S. mutans CFU 时，第 3 组和第 4 组之间没有差异（p>0.05），但其他组之间有差异（p<0.05）。当评价 S. mutans 的代谢活性时，第 1 组与其他组之间存在差异，第 2 组与第 5 组和第 6 组之间存在差异（p<0.05）。各组的 μTBS 值无差异（p>0.05）。结果表明，虽然通过绿色合成获得的 B-Ag NPs 和 B-Ag@nGO Ag NPs 的抗菌活性低于化学合成获得的 C-Ag NPs 和 C-Ag@nGO NCs，但与对照组相比，它们提供了更高的抗菌活性，并且不会降低 μTBS。将生物合成的 Ag NPs 添加到胶粘剂系统中通过保持胶粘剂的结合强度来增加抗菌效果。抗菌胶粘剂可以通过保护牙-胶粘剂界面来增加修复体的寿命。
