Sanyi Road, Changsha, Hunan Province, People's Republic of China.
Center for Genetic Medicine Research, Children's National Hospital. 111 Michigan Ave NW, Washington, DC 20010, USA.
Nucleic Acids Res. 2021 Jan 8;49(D1):D848-D854. doi: 10.1093/nar/gkaa809.
High-throughput genetic screening based on CRISPR/Cas9 or RNA-interference (RNAi) enables the exploration of genes associated with the phenotype of interest on a large scale. The rapid accumulation of public available genetic screening data provides a wealth of knowledge about genotype-to-phenotype relationships and a valuable resource for the systematic analysis of gene functions. Here we present CRISP-view, a comprehensive database of CRISPR/Cas9 and RNAi screening datasets that span multiple phenotypes, including in vitro and in vivo cell proliferation and viability, response to cancer immunotherapy, virus response, protein expression, etc. By 22 September 2020, CRISP-view has collected 10 321 human samples and 825 mouse samples from 167 papers. All the datasets have been curated, annotated, and processed by a standard MAGeCK-VISPR analysis pipeline with quality control (QC) metrics. We also developed a user-friendly webserver to visualize, explore, and search these datasets. The webserver is freely available at http://crispview.weililab.org.
基于 CRISPR/Cas9 或 RNA 干扰 (RNAi) 的高通量基因筛选能够大规模探索与感兴趣表型相关的基因。公共可用的遗传筛选数据的快速积累提供了丰富的基因型-表型关系知识，是系统分析基因功能的宝贵资源。在这里，我们介绍了 CRISP-view，这是一个综合的 CRISPR/Cas9 和 RNAi 筛选数据集数据库，涵盖了多种表型，包括体外和体内细胞增殖和活力、对癌症免疫疗法的反应、病毒反应、蛋白质表达等。截至 2020 年 9 月 22 日，CRISP-view 从 167 篇论文中收集了 10321 个人类样本和 825 个小鼠样本。所有数据集都经过了精心整理、注释，并通过标准的 MAGeCK-VISPR 分析管道进行了处理，具有质量控制 (QC) 指标。我们还开发了一个用户友好的网络服务器，用于可视化、探索和搜索这些数据集。该网络服务器可免费在 http://crispview.weililab.org 上获取。
