Detection of Tn-Like Transposons and Antibiotic Resistance in From Animals Used for Food Production With Identification of Three Novel Transposons Tn, Tn, and Tn.

are widely distributed in the gastro-intestinal system of animals and may cause opportunistic infections. Worse still, multidrug-resistant also poses a serious threat to public health. Tn-like transposons have been found in several species of the order and play an important role in dissemination of antibiotic resistance. This study aimed to investigate the distribution and genetic characterization of Tn-like transposons in isolates from food animals and their association with antibiotic resistance. isolated from the samples were identified and classified according to the 16S rDNA sequence. Tn-like transposons and associated integrons were detected by polymerase chain reaction (PCR) and sequencing. The antibiotic resistance of each Tn-like transposon positive isolate was detected according to the Kirby-Bauer disk diffusion method. Then, six representative strains were selected to study the genetic environment by whole-genome sequencing (WGS). In total, we isolated 377 Tn-like transposons positive strains of . Class 2 integrons were detected in 99.5% of the isolates, and there were high frequency mutation sites especially in base 535, a stop mutation. Many isolates (54.9%) were multidrug-resistant and observed high resistance rates to trimethoprim/sulfamethoxazole and streptomycin. Among these strains, we found three new types of Tn-like transposons, named Tn, Tn, and Tn. This is the first comprehensive survey that shows Tn-like transposons in from animals used for food production in different regions of China. This study also provides an insight into the horizontal transfer of resistance genes associated with Tn-like transposons.