Jiang Lingli, Tan Jingjie, Hao Yi, Wang Qi, Yan Xiaorui, Wang Dali, Tuo Li, Wei Zairong, Huang Guangtao
Department of Burn and Plastic, Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Life Sciences Institute, Zunyi Medical University, Zunyi, China.
Front Microbiol. 2020 Sep 8;11:506068. doi: 10.3389/fmicb.2020.506068. eCollection 2020.
has emerged as one of the most troublesome pathogens in health care institutions. can cause a wide range of diseases in humans, including pneumonia and septicemia. Phage therapy has drawn great interest from medical researchers as a potential way to control infections by antibiotic-resistant . Using a pandrug-resistant clinical isolate AB9 as an indicator, we isolated a lytic phage Abp9 from hospital sewage. Abp9 belongs to family and shows a wider host range of 12%. Abp9 contains a linear double-stranded DNA genome of 44,820 bp with a G + C content of 37.69%. The Abp9 genome contains 80 open reading frames, but lacks any known virulence genes or lysogen-formation genes. In a systemic infection mouse models, Abp9 treatment showed good therapeutic effects. We have also observed an excellent lytic activity against in biofilm form of growth . All of these suggest that Abp9 is a good candidate for the phage therapy against drug-resistant infections.
已成为医疗机构中最麻烦的病原体之一。可导致人类多种疾病，包括肺炎和败血症。噬菌体疗法作为一种控制耐抗生素感染的潜在方法，已引起医学研究人员的极大兴趣。以一株泛耐药临床分离株AB9为指示菌，我们从医院污水中分离出一种裂解性噬菌体Abp9。Abp9属于 科，宿主范围较广，为12%。Abp9含有一个44,820 bp的线性双链DNA基因组，G + C含量为37.69%。Abp9基因组包含80个开放阅读框，但缺乏任何已知的毒力基因或溶原形成基因。在系统性感染小鼠模型中，Abp9治疗显示出良好的治疗效果。我们还观察到Abp9对生物膜形式生长的 具有优异的裂解活性。所有这些表明Abp9是抗耐药 感染噬菌体疗法的良好候选者。
