Oubihi Asmaa, Hosni Hanae, Nounah Issmail, Ettouil Abdessamad, Harhar Hicham, Alaoui Katim, Ouhssine Mohammed, Guessous Zineb
Laboratory of Agro-Physiology, Biotechnology, Environment and Quality, Faculty of Science, Ibn Tofail University, Kenitra, Morocco.
Pharmacodynamy Research Team ERP, Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.
Biochem Res Int. 2020 Sep 18;2020:8823209. doi: 10.1155/2020/8823209. eCollection 2020.
is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.
是一种属于唇形科的药用植物，原产于摩洛哥，用于传统医学。本研究旨在研究地上部分两种乙醇提取物和甲醇提取物中的酚类化合物、抗氧化活性、抗炎作用及毒性。甲醇提取物的得率（22.2%）高于乙醇提取物（15.8%），其特点是多酚含量较高，为243.08毫克没食子酸当量/克（mg/g GAE）、黄酮类化合物含量为179.28毫克芦丁当量/克（mg/g RE）、单宁含量为39.31毫克儿茶素当量/克（mg/g CE）。通过2,2-二苯基-1-苦基肼（DPPH）自由基还原试验和特罗克斯当量抗氧化能力（TEAC）试验测定抗氧化活性，结果表明甲醇提取物的性能更高。对成年Wistar雌性大鼠的抗炎作用评估显示，与标准药物（吲哚美辛）和对照组相比，水肿炎症显著减轻。毒性试验表明，两种提取物在大鼠体重LD50高于2000毫克/千克时均无毒性。
