Boualam K, Ndiaye B, Harhar H, Tabyaoui M, Ayessou N, Taghzouti K
Laboratory of Materials, Nanotechnology and Environment, Faculty of Sciences-Rabat, Mohammed V University of Rabat, BP 1014-Rabat, Morocco.
Genomics of Human Pathologies Research Center, Faculty of Sciences, Mohammed V University in Rabat, BP1014 Rabat, Morocco.
Adv Pharmacol Pharm Sci. 2021 May 4;2021:5549478. doi: 10.1155/2021/5549478. eCollection 2021.
The aim of this study is to perform phytochemical screening of the leaves of and , to determine the phenolic, flavonoids, tannins, and sugars content in their methanolic extracts, evaluate their antioxidant activity using the DPPH and the ABTS tests, and test their anti-inflammatory effect in vitro using the heat-induced albumin denaturation inhibition method. Phytochemical screening revealed the presence of polyphenols and alkaloids in the leaves of both plants. Yields of the extracts in this study ranged from 7% to 18% for and 4% to 16% for . The phenolic content in the methanolic extract of is 74.66 ± 1.76 g GAE/mL, which is significantly higher than that of (56.54 ± 1.24 g GAE/mL). Both plants showed good antioxidant activity. In fact, for the DPPH test, the IC50 value is 8.88 ± 0.11 g/mL for and 17.64 ± 0.68 g/mL for . For the ABTS assay, the IC values of the two plants are, respectively, 9.78 ± 1.83 g/mL and 13.47 ± 2.62 g/ml, statistically comparable and significantly higher than the IC of the standard 30.76 ± 0.18 g/ml. Leaf extracts from both plants were effective against heat-induced denaturation of albumin. The activity of is indeed comparable to that of the standard with an IC value of 121.43 ± 1.55 g/mL and higher than that of with an IC value of 170.15 ± 1.09 g/mL. These results show that both plants exhibit significant antioxidant and anti-inflammatory activities. Therefore, their chemical compounds could have potential applications as antioxidant and anti-inflammatory drugs.
本研究的目的是对[植物名称1]和[植物名称2]的叶子进行植物化学筛选，测定其甲醇提取物中的酚类、黄酮类、单宁和糖类含量，使用DPPH和ABTS试验评估其抗氧化活性，并采用热诱导白蛋白变性抑制法体外测试其抗炎作用。植物化学筛选显示两种植物的叶子中均存在多酚和生物碱。本研究中[植物名称1]提取物的产率为7%至18%，[植物名称2]提取物的产率为4%至16%。[植物名称1]甲醇提取物中的酚类含量为74.66±1.76 g GAE/mL，显著高于[植物名称2]（56.54±1.24 g GAE/mL）。两种植物均表现出良好的抗氧化活性。事实上，在DPPH试验中，[植物名称1]的IC50值为8.88±0.11 g/mL，[植物名称2]的IC50值为17.64±0.68 g/mL。在ABTS试验中，两种植物的IC值分别为9.78±1.83 g/mL和13.47±2.62 g/mL，在统计学上具有可比性，且显著高于标准品的IC值30.76±0.18 g/mL。两种植物的叶子提取物均对热诱导的白蛋白变性有效。[植物名称1]的活性确实与标准品相当，IC值为121.43±1.55 g/mL，高于[植物名称2]，其IC值为170.15±1.09 g/mL。这些结果表明两种植物均表现出显著的抗氧化和抗炎活性。因此，它们的化合物可能具有作为抗氧化和抗炎药物的潜在应用。
需注意，原文中部分植物名称未给出具体信息，我用[植物名称1]和[植物名称2]代替进行了翻译。
