Younis Sara H, Obeid Raneem F, Ammar Mohamed M
Lecturer of Conservative Dentistry, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, Egypt.
Lecturer of Oral Biology, Faculty of Oral and Dental Medicine, Future University in Egypt, Cairo, Egypt.
Heliyon. 2020 Sep 28;6(9):e05054. doi: 10.1016/j.heliyon.2020.e05054. eCollection 2020 Sep.
The objective of this study was to investigate enamel remineralization using lyophilized extract from Moringa Oleifera Leaves (MOL).
A total of seventy-five teeth were selected and randomly divided into 5 equal groups (n = 15). All teeth were air dried for 30 s, two windows (right and left) were drawn on the labial surface of each tooth. The right window was set as the control and no etching or treatment was performed. The left window was etched for 30 s for all five groups. After etching, group I received no treatment, group II received plain varnish, group III received fluoride varnish, and finally groups IV and V received plain varnish loaded with 50 mg/ml and 200 mg/ml of lyophilized MOL extract respectively. All samples were then immersed in artificial saliva for 14 days. The specimens were examined under scanning electron microscope (SEM) and energy dispersive x-ray (EDX).
SEM micrographs revealed that MOL extract loaded-varnish groups IV & V showed the most re-establishment of normal enamel architecture. Elemental analysis of the treated surfaces of Groups IV and V showed the surfaces treated by MOL loaded-varnish groups had significantly higher Ca, P, and O deposition than the fluoride varnish group.
MOL might be considered as a biomimetic material capable of guiding enamel tissue remineralization.
本研究的目的是调查使用辣木叶片冻干提取物（MOL）进行牙釉质再矿化的情况。
共选取75颗牙齿，随机分为5组（每组n = 15）。所有牙齿均在空气中干燥30秒，在每颗牙齿的唇面绘制两个窗口（右侧和左侧）。右侧窗口作为对照，不进行酸蚀或处理。所有五组的左侧窗口均酸蚀30秒。酸蚀后，第一组不进行处理，第二组接受普通清漆，第三组接受氟化物清漆，最后第四组和第五组分别接受负载50mg/ml和200mg/ml辣木叶片冻干提取物的普通清漆。然后将所有样品浸入人工唾液中14天。在扫描电子显微镜（SEM）和能量色散X射线（EDX）下检查标本。
扫描电子显微镜照片显示，负载辣木叶片冻干提取物的清漆组（第四组和第五组）显示出正常牙釉质结构的最大重建。第四组和第五组处理表面的元素分析表明，负载辣木叶片冻干提取物的清漆组处理的表面比氟化物清漆组具有明显更高的钙、磷和氧沉积。
辣木叶片冻干提取物可被视为一种能够引导牙釉质组织再矿化的仿生材料。
